AN IMMUNOCYTOCHEMICAL ANALYSIS OF THE ONTOGENY OF THE MICROTUBULE-ASSOCIATED PROTEINS MAP-2 AND TAU IN THE NERVOUS-SYSTEM OF THE RAT
AN IMMUNOCYTOCHEMICAL ANALYSIS OF THE ONTOGENY OF THE MICROTUBULE-ASSOCIATED PROTEINS MAP-2 AND TAU IN THE NERVOUS-SYSTEM OF THE RAT
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DOI：
10.1016/0165-3806(87)90191-x
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发表时间：
1987-07-01
期刊：
影响因子：
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通讯作者：
CACERES, A
中科院分区：
文献类型：
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作者：
FERREIRA, A;BUSCIGLIO, J;CACERES, A
β的发育分布模式-微管蛋白和微管相关蛋白，MAP-2和Tau，用单克隆抗体的免疫细胞化学进行了研究。对这些蛋白质在胚胎脑组织中的原位分布的分析揭示了β-微管蛋白在增殖和迁移的神经母细胞。相反，在该神经上皮中未检测到MAP-2或Tau的免疫反应性;这些MAP的特异性免疫染色仅存在于已在发育中的脑区域内到达其最终目的地的那些成神经细胞中，并且已启动终末分化，即轴突和树突的发芽。在神经炎生长的初始阶段，在发育中的脑神经元的体树突隔室中检测到两种MAP; Tau也存在于轴突中。虽然MAP-2的分布在整个发育过程中基本保持不变，但Tau从细胞体和树突中逐渐丢失。在大脑皮层和海马的锥体神经元以及其他脑区（例如丘脑、下丘脑、大脑杏仁核和顶盖）的细胞中观察到这种区室化模式。在小脑浦肯野细胞中未检测到，该细胞从神经炎分化的开始将Tau区室化到轴突，并且在Gasser神经节的神经元中未检测到，该神经元在轴突中瞬时表达MAP-2。这些MAP的表达和分布也进行了分析，在胚胎小脑和海马锥体神经元的培养。这两种MAP在这些细胞中发现后6小时，他们也存在于所有的神经突，轴突和树突，这些细胞在体外发育后延伸数天。随着随后的发育（体外超过4天），MAP-2从轴突中丢失，而Tau仍然均匀分布在两种类型的神经突中。总的来说，本研究结果表明，MAP-2和Tau的区室化分布的发展遵循复杂的模式，该模式对这些MAP中的每一种都是特异性的，并且作为神经元类型和细胞发育条件的函数而变化。此外，在原位和体外发育过程中，两种MAP的分布的复杂变化使得这些蛋白质不太可能在决定神经突作为轴突或树突的命运中发挥作用。
The developmental distribution patterns of .beta.-tubulin and the microtubule-associated proteins, MAP-2 and Tau, were studied by immunocytochemistry with monoclonal antibodies. The analysis of the in situ distribution of these proteins in embryonic brain tissue revealed intense immunoreactivity for .beta.-tubulin in proliferative and migrating neuroblasts. On the contrary, no immunoreactivity for MAP-2 or Tau was detected in this neuroepithelium; specific immunostaining for these MAPs was only present in those neuroblasts which have reached their final destination within a developing brain area, and have initiated terminal differentiation, i.e. the sprouting of axons and dendrites. During the initial stages of neuritic outgrowth both MAPs were detected in the somatodendritic compartment of developing brain neurons; Tau was also present in axons. While the distribution of MAP-2 remained essentially the same throughout development, Tau was progressively lost from cell bodies and dendrites. This pattern of compartmentation was observed in pyramidal neurons of the cerebral cortex and hippocampus, as well as in cells of other brain regions (e.g. thalamus, hypothalamus, cerebral amygdala and tectum). It was not detected in cerebellar Purkinje cells which compartmentalize Tau to axons from the outset of neuritic differentiation, and in neurons of the Gasser ganglion which transiently express MAP-2 in axons. The expression and distribution of these MAPs was also analyzed in embryonic cerebellar and hippocampal pyramidal neurons grown in culture. Both MAPs were found in these cells as soon as 6 h after plating; they were also present in all of the neurites, axons and dendrites, that these cells extend after development in vitro for several days. With subsequent development (more than 4 days in vitro) MAP-2 was lost from axons, while Tau remained homogeneously distributed in both types of neurites. Taken collectively, the present results indicate that the development of the compartmentalized distribution of MAP-2 and Tau follows a complex pattern which is specific for each of these MAPs, and which varies as a function of the neuron type and the conditions under which the cell develops. In addition, the complex variations in the distribution of both MAPs during in situ and in vitro development make it unlikely that these proteins have a role in determining the fate of a neurite as an axon or a dendrite.