Absolute morphometric study of myocardial hypertrophy in experimental hypertension. I. Determination of myocyte size.
Absolute morphometric study of myocardial hypertrophy in experimental hypertension. I. Determination of myocyte size.
复制标题
实验性高血压心肌肥厚的绝对形态测量研究。
DOI：
--
复制
发表时间：
1978
期刊：
影响因子：
--
通讯作者：
Joseph Wiener
中科院分区：
文献类型：
--
作者：
Loud Av;Piero Anversa;Filiberto Giacomelli;Joseph Wiener
本文描述了一种测定组织内特定细胞群的平均绝对体积的方法，并应用于正常和高血压大鼠左心室内膜和心外膜肌细胞的测量。该技术基于不同已知厚度的组织切片中每单位面积的细胞核计数，测量每个细胞核的平均细胞体积，而不依赖于先前未知的细胞核尺寸和系统计数误差。在心肌的相互垂直的纵向和横向切片中进行重复测定，证明具有重现性。将这些光学显微镜测量与电子显微镜数据相结合，能够评估圆柱形肌细胞核的平均直径和长度，显示心外膜细胞中的细胞核明显长于内膜细胞中的细胞核。据估计，2%至4%的心室肌细胞是双核细胞。在左肾动脉收缩引起的高血压1至4周后，内皮细胞扩大了21%，从10，370 +/- 410增加到12，520 +/- 490 cu。微米，而心外膜肌细胞显示出37%的肥大，从12，600 +/-1，600到17，300 +/-1，100 cu。千分尺。细胞体积的可靠测定的可用性将使得在整个细胞水平上解释许多生物化学、功能和形态学数据成为可能。
A method for determining the mean absolute volume of a specific population of cells within a tissue is described and applied to the measurement of endocardial and epicardial myocytes in the left ventricle of normal and hypertensive rats. The technique, based on nuclear counts per unit area in tissue slices of different known thicknesses, measures the mean cell volume per nucleus independent of previously unknown nuclear dimensions and systematic counting errors. Duplicate determinations, demonstrating reproducibility, were made in mutually perpendicular longitudinal and transverse sections of the myocardium. Combining these light microscopic measurements with electron microscopic data enabled the evaluation of the mean diameter and length of the cylindrical myocyte nuclei showing those in the epicardial cells to be significantly longer than the nuclei in endocardial cells. It was estimated that 2 to 4 per cent of ventricular myocytes are binucleate. After 1 to 4 weeks of hypertension, induced by constriction of the left renal artery, endocardial myocytes were enlarged 21 per cent, from 10,370 +/- 410 to 12,520 +/- 490 cu. micrometer., while epicardial myocytes showed a 37 per cent hypertrophy, from 12,600 +/- 1,600 to 17,300 +/- 1,100 cu. micrometer. The availability of a reliable determination of cell volume will make possible the interpretation of much biochemical, functional, and morphometric data at the whole cell level.