Association of RON tyrosine kinase with the Jaagsiekte sheep retrovirus envelope glycoprotein.
Association of RON tyrosine kinase with the Jaagsiekte sheep retrovirus envelope glycoprotein.
复制标题
RON 酪氨酸激酶与 Jaagsiekte 绵羊逆转录病毒包膜糖蛋白的关联。
DOI：
10.1016/j.virol.2006.01.040
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发表时间：
2006
期刊：
影响因子：
3.7
通讯作者：
Palmarini,Massimo
中科院分区：
文献类型：
--
作者：
Varela,Mariana;Chow,Yen-Hung;Sturkie,Carla;Murcia,Pablo;Palmarini,Massimo
绵羊肺腺瘤病毒（Jaagsiekte sheep retrovirus，JSRV）的包膜蛋白（Env）是一种癌蛋白。已经提出的用于上皮细胞的JSRV诱导的细胞转化的机制之一涉及JSRV Env结合Hybryonidase 2（JSRV受体），从而诱导其降解并允许释放和激活通常被HYAL-2抑制的罗恩酪氨酸激酶。在这项研究中，我们报告说，HYAL-2和罗恩是不是关键的JSRV Env诱导转化的大鼠上皮细胞系IEC-18，而细胞质尾的JSRV Env是关键的转化该细胞系。我们还确定了罗恩在正常和严格条件下可以与JSRV Env结合。此外，JSRV和enJS 5 F16（非致癌性JSRV相关内源性逆转录病毒）Env蛋白的胞质尾区似乎对罗恩的活化状态具有主要影响。因此，看来JSRV Env与罗恩的相互作用比以前认为的更复杂，需要进一步研究。
The envelope (Env) of Jaagsiekte sheep retrovirus (JSRV) functions as an oncoprotein. One of the mechanisms of JSRV-induced cell transformation that has been proposed for epithelial cells involves JSRV Env binding Hyaluronidase 2 (the JSRV receptor), thereby inducing its degradation and allowing the release and activation of RON tyrosine kinase which is normally suppressed by HYAL-2. In this study, we report that HYAL-2 and RON are not critical for the JSRV Env-induced transformation of the rat epithelial cell line IEC-18, while the cytoplasmic tail of the JSRV Env is critical to transform this cell line. We have also determined that RON can associate with the JSRV Env under normal and stringent conditions. In addition, the cytoplasmic tail of the JSRV and the enJS5F16 (non oncogenic JSRV-related endogenous retrovirus) Env proteins appears to have a major influence on the activation status of RON. Thus, it appears that the interaction of the JSRV Env with RON is more complex than previously thought and requires further investigation.