CD44 VARIANT EXPRESSION IS A COMMON FEATURE OF EPITHELIAL OVARIAN-CANCER - LACK OF ASSOCIATION WITH STANDARD PROGNOSTIC FACTORS
CD44 VARIANT EXPRESSION IS A COMMON FEATURE OF EPITHELIAL OVARIAN-CANCER - LACK OF ASSOCIATION WITH STANDARD PROGNOSTIC FACTORS
复制标题
DOI：
10.1200/jco.1995.13.8.1912
复制
发表时间：
1995-08-01
影响因子：
45.3
通讯作者：
OTTENSMEIER, C
中科院分区：
文献类型：
--
作者：
CANNISTRA, SA;ABUJAWDEH, G;OTTENSMEIER, C
目的：CD44是一种透明质酸受体，作为标准的90 kD形式（CD44S）以及通过替代剪接产生的几种CD44变体同工型。 CD44变体的表达与某些人类肿瘤的临床侵略行为有关。本研究的目的是定义卵巢癌中CD44变体同工型的表达，并研究这些分子的表达是否与不良预后相关。材料和方法六个正常卵巢表面上皮（鼻子）和31个单独病例的六个样本通过反向转录聚合酶链反应（RT-PCR）和免疫过氧化物酶染色。 CD44变异表达和期（I/II V III/IV），残留疾病（小于或等于2.0- V> 2.0 cm质量），年龄（小于或等于65 V> 65年）之间产生的临床相关性WET（I/II V III/IV） ），组织学（乳头状浆液v其他），等级和生存。分析：RT-PCR分析表明，鼻子主要表达CD44的转录本，以及受限制CD44剪接变体的转录本特征的模式。 CD44和CD44变体外显子9序（CD44-9V）在通过免疫过氧化物酶染色检查的两个鼻子样本之一中焦点表达。相比之下，大多数卵巢癌标本（71％）通过RT-PCR分析表达了CD44剪接变体的复杂模式。免疫过氧化物酶的研究表明，大多数卵巢癌标本同时表达CD44S和CD44-9V，而变体外显子3、4和6的序列表达并不常见。 CD44变异表达（转录或蛋白质）与阶段，残留疾病，年龄，组织学，等级或生存之间没有关联。结论：CD44S和CD44-9V的表达是上皮卵巢癌细胞的共同特征。 CD44变体表达和预后之间缺乏显着关联表明，其他因素在确定该疾病的临床行为方面可能更为重要。JClin Oncol 13：1912-1921。 （c）1995年美国临床肿瘤学会。
Purpose: CD44 is a hyaluronic acid receptor that exists as a standard 90-kd form (CD44S) as well as several CD44 variant isoforms produced through alternative splicing. Expression of CD44 variants is associated with clinically aggressive behavior in some human tumors. The purpose of the present study is to define the expression of CD44 variant isoforms in ovarian cancer and to investigate whether the expression of these molecules is associated with adverse prognosis.Materials and Methods Six specimens of normal ovarian surface epithelium (NOSE) and 31 separate cases of newly diagnosed ovarian cancer were studied by a combination of reverse-transcription polymerase chain reaction (RT-PCR) and immunoperoxidase staining. Clinical correlation wets made between CD44 variant expression and stage (I/II v III/IV), residual disease (less than or equal to 2.0- v > 2.0-cm mass), age (less than or equal to 65 v > 65 years), histology (papillary serous v other), grade, and survival.Results: RT-PCR analysis revealed that NOSE predominantly expressed transcripts for CD44S, as well as a restricted pattern of transcripts characteristic of CD44 splice variants. CD44S and CD44 variant exon nine sequences (CD44-9v) were focally expressed in one of two NOSE specimens examined by immunoperoxidase staining. In comparison, the majority (71%) of ovarian cancer specimens expressed a complex pattern of CD44 splice variants by RT-PCR analysis. Immunoperoxidase studies revealed that the majority of ovarian cancer specimens expressed both CD44S and CD44-9v, whereas expression of sequences from variant exons 3, 4, and 6 was uncommon. There was no association between CD44 variant expression (transcript or protein) and stage, residual disease, age, histology, grade, or survival.Conclusion: Expression of CD44S and CD44-9v is a common feature of epithelial ovarian cancer cells. The lack of a significant association between CD44 variant expression and prognosis suggests that other factors may be more important in determining the clinical behavior of this disease.J Clin Oncol 13:1912-1921. (C) 1995 by American Society of Clinical Oncology.