GDI1 ENCODES A GDP DISSOCIATION INHIBITOR THAT PLAYS AN ESSENTIAL ROLE IN THE YEAST SECRETORY PATHWAY
GDI1 ENCODES A GDP DISSOCIATION INHIBITOR THAT PLAYS AN ESSENTIAL ROLE IN THE YEAST SECRETORY PATHWAY
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DOI：
10.1002/j.1460-2075.1994.tb06436.x
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发表时间：
1994-04-01
期刊：
影响因子：
11.4
通讯作者：
NOVICK, PJ
中科院分区：
文献类型：
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作者：
GARRETT, MD;ZAHNER, JE;NOVICK, PJ
Sec 4/Ypt/rab家族的GTP结合蛋白调节真核细胞中不同的囊泡交通事件。我们已经克隆了GD 11，一个必需的同源物的牛rab GDI（GDP解离抑制剂）从酵母酿酒酵母。与牛蛋白类似，纯化的Gdi 1 p减缓了GDP从Sec 4p的解离，并从酵母膜释放GDP结合形式。体内Gdi 1 p的消耗导致Sec 4p的可溶性库的损失和在分泌途径的多个阶段的蛋白质转运的抑制。互补分析表明GD 11与sec 19 -1等位。这些结果表明，Gdi 1 p在膜交通中起着重要的作用，并且与Gdi 1 p在Sec 3/Ypt/rab家族的蛋白质从其靶膜回收回其囊泡池中的作用一致。
GTP binding proteins of the Sec4/Ypt/rab family regulate distinct vesicular traffic events in eukaryotic cells. We have cloned GD11, an essential homolog of bovine rab GDI (GDP dissociation inhibitor) from the yeast Saccharomyces cervisiae. Analogous to the bovine protein, purified Gdi1p slows the dissociation of GDP from Sec4p and releases the GDP-bound form from yeast membranes. Depletion of Gdi1p in vivo leads to loss of the soluble pool of Sec4p and inhibition of protein transport at multiple stages of the secretory pathway. Complementation analysis indicates that GD11 is allelic to sec19-1. These results establish that Gdi1p plays an essential function in membrane traffic and are consistent with a role for Gdi1p in the recycling of proteins of the Sec3/Ypt/rab family from their target membranes back to their vesicular pools.