Differential expression and clinical significance of glioblastoma mRNA expression profiles in Uyghur and Han patients in Xinjiang province.
Differential expression and clinical significance of glioblastoma mRNA expression profiles in Uyghur and Han patients in Xinjiang province.
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DOI：
10.12659/msm.892519
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发表时间：
2014-11-24
期刊：
影响因子：
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通讯作者：
Luan X
中科院分区：
文献类型：
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作者：
Liu L;Li W;Xia H;Zhu Z;Luan X
本研究旨在探讨新疆维吾尔族和汉族胶质母细胞瘤RNA基因表达谱的差异，筛选和比较差异表达基因在高级别胶质瘤发病机制中的临床意义及其与疾病预后的关系。采用Illumina HT-12mRNA表达谱芯片测定6例晚期胶质瘤患者的基因表达谱并筛选差异表达基因。采用Web Gestalt软件对差异表达基因进行GO和KEGG分析（P<0.05）。比较维吾尔族和汉族患者胶质母细胞瘤RNA表达谱，发现1475个基因表达存在显着差异，其中669个基因表达增加，807个基因表达减少。一个基因（STRC）对应2个转录本，其中1个显示表达增加，另一个显示表达减少。差异表达基因参与代谢过程、生物调节、应激反应和多细胞有机过程，包括小GTPase调节信号通路、Ras信号通路、神经元反应蛋白调节和中枢神经系统髓鞘形成。这些基因还参与肿瘤相关信号通路，包括代谢通路、癌症通路、MAPK信号通路、TGF-β信号通路、神经营养因子信号转导通路、mTOR信号通路等。通过研究维吾尔族和汉族胶质母细胞瘤基因表达谱来筛选差异表达基因。进一步研究了这些基因的细胞功能和位置。基于胶质母细胞瘤相关分子标志物，探讨维吾尔族和汉族胶质母细胞瘤发生和发展机制的差异，探讨多基因相互作用。
The aim of this study was to investigate differences in glioblastoma RNA gene expression profiles between Uyghur and Han patients in Xinjiang province and to screen and compare differentially expressed genes with respect to their clinical significance in the pathogenesis of high-grade glioma and their relationship to disease prognosis. Illumina HT-12mRNA expression profiles microarray was employed to measure the gene expression profiles of 6 patients with advanced glioma and to screen for differentially expressed genes. GO and KEGG analyses were performed on the differentially expressed genes using Web Gestalt software (P<0.05). Comparison of glioblastoma RNA expression profiles in the Uyghur and Han patients indicated that 1475 genes were significantly differentially expressed, of which 669 showed increased expression, while 807 showed decreased expression. One gene (STRC) corresponded to 2 transcripts, 1 of which showed increased expression and the other showed decreased expression. The differentially expressed genes participate in metabolic processes, biological regulation, stress response, and multi-cellular organic processes, including small GTPase regulatory signaling pathways, Ras signaling pathway, neuronal reactive protein regulation, and myelination of the central nervous system. The genes are also involved in tumor-related signaling pathways, including metabolic pathways, cancer pathways, MAPK signaling pathway, TGF-β signaling pathway, neurotrophic factor signal transduction pathway, and mTOR signaling pathway. Differentially expressed genes were screened by studying the gene expression profiles in glioblastoma from Uyghur and Han patients. The cellular function and location of these genes were further investigated. Based on related molecular markers of glioblastoma, the differences in the mechanism of initiation and development of glioblastoma between Uyghur and Han patients were investigated for polygenic interactions.