Screening for cytotoxic activity of extracts and isolated alkaloids from bulbs of Hippeastrum vittatum
Screening for cytotoxic activity of extracts and isolated alkaloids from bulbs of Hippeastrum vittatum
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DOI：
10.1016/j.phymed.2007.12.001
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发表时间：
2008-10-01
期刊：
影响因子：
7.9
通讯作者：
Zuanazzi, J. A. S.
中科院分区：
文献类型：
--
作者：
Silva, A. F. S.;de Andrade, J. P.;Zuanazzi, J. A. S.
采用磺基罗丹明B试验，评价了从巴西南部采集的朱顶红（石蒜科）新鲜鳞茎中获得的二氯甲烷和正丁醇提取物对5种人细胞系（HT 29结肠腺癌、H460非小细胞肺癌、RXF 393肾细胞癌、MCF 7乳腺癌和OVCAR 3上皮性卵巢癌）的体外细胞毒性活性。两种提取物均表现出潜在的抗增殖活性。从氯仿萃取物中分离得到石蒜碱、vittatine和montanine三种生物碱。对最后两种化合物进行抗增殖试验，发现生物碱蒙他宁的细胞毒性水平最高。(C)2007年，Elsevier GmbH。All rights reserved.
The dichloromethane and n-butanol extracts obtained from fresh bulbs of Hippeastrum vittatum (Amaryllidaceae), collected in Southern Brazil, were evaluated for their cytotoxic activity in vitro against five human cell lines (HT29 colon adenocarcinoma, H460 non-small cell lung carcinoma, RXF393 renal cell carcinoma, MCF7 breast cancer, and OVCAR3 epithelial ovarian cancer), using the sulphorhodamine B assay. Both extracts showed potential antiproliferative activity. From CH2Cl2 fraction, three alkaloids were isolated: lycorine, vittatine and montanine. The two last compounds were submitted to the antiproliferative assay and the highest level of cytotoxicity was found for the alkaloid montanine. (C) 2007 Elsevier GmbH. All rights reserved.