Osteoinductive Agents-Incorporated Three-Dimensional Biphasic Polymer Scaffold for Synergistic Bone Regeneration
Osteoinductive Agents-Incorporated Three-Dimensional Biphasic Polymer Scaffold for Synergistic Bone Regeneration
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结合骨诱导剂的三维双相聚合物支架用于协同骨再生
DOI：
10.1021/acsbiomaterials.8b01371
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发表时间：
2019
影响因子：
--
通讯作者：
Chen Xuesi
中科院分区：
文献类型：
--
作者：
Zhu Bitao;Xu Weiguo;Liu Jianguo;Ding Jianxun;Chen Xuesi
临床上大面积骨缺损难以完全再生。骨组织工程以其简便、灵活的优点作为骨移植的替代方法而受到越来越多的关注。然而，支架的低生物活性和生长因子的不利影响阻碍了其实际应用。本文中，聚（乳酸-共-乙醇酸）（PLGA）支架的性能，包括亲水性和机械强度，通过明胶涂层与两个小分子，阿仑膦酸钠（ALD）和柚皮苷（NG）的掺入得到改善。有趣的是，这两种药物对大鼠颅骨缺损的修复表现出协同作用，因为ALD对破骨细胞活性具有抑制作用，NG对间充质干细胞具有成骨作用。从组织病理学染色和显微计算机断层扫描的结果，PLGA支架与明胶，ALD，NG（PLGA+明胶/ALD/NG）纳入几乎完全修复大鼠颅骨缺损的生理完整性在16周。总之，这种双相支架可以是用于临床应用的常规支架的有希望的替代物。
Large-scale bone defects are difficult to be regenerated entirely in the clinical practice. Bone tissue engineering has drawn more attention as an alternative to bone grafting owing to its convenience and flexibility. However, the low bioactivity of scaffolds and adverse effects of growth factors have hindered its practical application. Herein, the properties of poly(lactic-co-glycolic acid) (PLGA) scaffold, including hydrophilicity and mechanical strength, were improved by a gelatin coating incorporated with two small molecules, alendronate (ALD) and naringin (NG). Interestingly, these two drugs demonstrated a synergistic effect for the repair of rat calvarial defect, as ALD had an inhibitory impact on osteoclast activity and NG had an osteogenic effect on mesenchymal stem cells. From the results of histopathological staining and microcomputed tomography, the PLGA scaffold incorporated with gelatin, ALD, and NG (PLGA+Gelatin/ALD/NG) almost completely repaired the rat calvarial defect with physiological integrity at 16 weeks. In all, this biphasic scaffold can be a promising alternative to the conventional scaffold for clinical application.