A genomic analysis of the shade avoidance response in Arabidopsis
A genomic analysis of the shade avoidance response in Arabidopsis
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DOI：
10.1104/pp.103.034397
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发表时间：
2003-12-01
期刊：
影响因子：
7.4
通讯作者：
Kay, SA
中科院分区：
文献类型：
--
作者：
Devlin, PF;Yanovsky, MJ;Kay, SA
植物对邻近植被的反应是伸长以防止遮荫。从邻近植被反射的红色耗尽光引发了避荫反应，导致植物结构发生了巨大变化。光质量的这些变化由光敏色素家族的光感受器检测到。我们分析了全球变化的基因表达随着时间的推移，野生型，phyB突变体，phyA phyB双突变体的拟南芥幼苗在模拟遮荫。使用模式拟合软件，我们确定了301个基因的表达模式对应于各种生理反应模式之一的阴影响应。以这种方式要求在12个芯片上的表达模式一致，使得基因表达中更微妙的变化被认为是有意义的。一些先前表征的基因参与光和激素信号被确定为阴影响应，以及几个推定的，新的阴影特异性信号转导因子。此外，还观察到与伸长生长和应激反应相关的一系列途径中基因表达的变化。大多数的遮荫响应基因表现出拮抗调节phyA和phyB在响应阴影以下的许多生理反应的模式。以这种方式调节的基因的启动子元件的分析确定了保守的启动子基序可能是重要的遮荫调节。
Plants respond to the proximity of neighboring vegetation by elongating to prevent shading. Red-depleted light reflected from neighboring vegetation triggers a shade avoidance response leading to a dramatic change in plant architecture. These changes in light quality are detected by the phytochrome family of photoreceptors. We analyzed global changes in gene expression over time in wild-type, phyB mutant, and phyA phyB double mutant seedlings of Arabidopsis in response to simulated shade. Using pattern fitting software, we identified 301 genes as shade responsive with patterns of expression corresponding to one of various physiological response modes. A requirement for a consistent pattern of expression across 12 chips in this way allowed more subtle changes in gene expression to be considered meaningful. A number of previously characterized genes involved in light and hormone signaling were identified as shade responsive, as well as several putative, novel shade-specific signal transduction factors. In addition, changes in expression of genes in a range of pathways associated with elongation growth and stress responses were observed. The majority of shade-responsive genes demonstrated antagonistic regulation by phyA and phyB in response to shade following the pattern of many physiological responses. An analysis of promoter elements of genes regulated in this way identified conserved promoter motifs potentially important in shade regulation.