Rapid selection of sperm with high DNA integrity
Rapid selection of sperm with high DNA integrity
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DOI：
10.1039/c3lc51254a
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发表时间：
2014-01-01
期刊：
影响因子：
6.1
通讯作者：
Sinton, David
中科院分区：
文献类型：
--
作者：
Nosrati, Reza;Vollmer, Marion;Sinton, David
精子选择对辅助生殖技术（ART）至关重要，影响治疗结果和后代的健康。精子选择的基本挑战是由生物学决定的：在体外，每毫升大约有10(8)个精子的异质群体，寿命很短。然而，传统的精子选择方法导致不到50%的DNA完整性改善。本文提出了一种临床应用的微流控装置，该装置基于500个平行微通道中的渐进运动来选择精子。结果是在20分钟内从1毫升生精液中进行精液纯化和高DNA完整性精子选择的一步程序。公牛精子的实验表明，选择的精子活力提高了89%以上。对人类精子的临床试验表明，人类DNA完整性提高了80%以上，显著优于目前的最佳做法。这些结果表明，存在一种几乎完整的染色质和DNA完整性的精子亚群，以及一种简单的临床适用的芯片实验室方法来选择这一群体。
Sperm selection is essential to assisted reproductive technology (ART), influencing treatment outcomes and the health of offspring. The fundamental challenge of sperm selection is dictated by biology: a heterogeneous population of similar to 10(8) sperm per milliliter with a short lifetime in vitro. However, conventional sperm selection approaches result in less than 50% improvement in DNA integrity. Here, a clinically applicable microfluidic device is presented that selects sperm based on the progressive motility in 500 parallel microchannels. The result is a one-step procedure for semen purification and high DNA integrity sperm selection from 1 mL of raw semen in under 20 minutes. Experiments with bull sperm indicate more than 89% improvement in selected sperm vitality. Clinical tests with human sperm show more than 80% improvement in human DNA integrity, significantly outperforming the best current practices. These results demonstrate the presence of a sub-population of sperm with nearly intact chromatin and DNA integrity, and a simple clinically-applicable lab-on-a-chip method to select this population.