ENVIRONMENTAL EFFECTS ON VIBRONIC BAND INTENSITIES IN PYRENE MONOMER FLUORESCENCE AND THEIR APPLICATION IN STUDIES OF MICELLAR SYSTEMS
ENVIRONMENTAL EFFECTS ON VIBRONIC BAND INTENSITIES IN PYRENE MONOMER FLUORESCENCE AND THEIR APPLICATION IN STUDIES OF MICELLAR SYSTEMS
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DOI：
10.1021/ja00449a004
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发表时间：
1977-01-01
影响因子：
15
通讯作者：
THOMAS, JK
中科院分区：
文献类型：
--
作者：
KALYANASUNDARAM, K;THOMAS, JK
结论研究了不同溶剂对呋喃霉素酰胺和酯C=0伸缩频率的影响。以固体构象D的拉曼光谱为参照，对酰胺和酯C=0基团的氢键程度作出了初步的结论。我们发现有证据表明，在溶于非极性溶剂的VM中，可能存在氢键酯C=0基团；这反过来可能表明构象D是存在的构象之一。增加溶剂的极性将VM的构象平衡转移到含有较少氢键的形式；这些结果与使用其他技术的发现一致。8-11溶剂的VCO与介电常数的关系图显示，C=0基团的VCO增加，而酯C=0基团的VCO减少。这可能反映了极性或氢键溶剂引起的酰胺C=0氢键的减弱或断裂，以及伴随而来的溶剂与酯C=0基团相互作用的增加。进一步的拉曼光谱研究将对双分子膜中的VM进行研究，就像最近对膜蛋白视蛋白所做的那样。27认知度。我们感谢与RC Lord博士、WL Duax博士、B.C.Pressman博士、G.Phillie博士、B.Tokar博士、Andr博士的有益讨论。桑切斯。这项工作得到了NEI的支持
ConclusionThis study has focussed on the effect of different solvents on the amide and ester C= 0 stretch frequencies of valinomycin. Using the Raman spectrum of the solid-state con-formation D as a reference, tentative conclusions were drawn about the extent of hydrogen bonding of the amide and ester C= 0 groups. We find evidence suggesting that hydrogen-bonded ester C= 0 groups may be present in VM dissolved in nonpolar solvents; this in turn may indicate that conformer D is among the conformers present. Increasing the polarityof the solvent shifts the conformational equilibrium ofVM to forms containing fewer hydrogen bonds; these results are consistent with findings using other techniques. 8-11 A plot of vco vs. dielectric constant of the solvent reveals an increase in vco fr amide C= 0 groups and a decrease in vco for ester C= 0 groups. This mayreflect weakening or rupture of amide C= 0 hydrogen bonds induced by polar or hydrogen bonding solvents and a concomitant in-crease in solvent interaction with ester C= 0 groups. Further Raman studies will be made of VM incorporated into bilayers, as has recently been done for the membrane protein opsin. 27Acknowledgments. We acknowledge helpful discussions with Drs. RC Lord, WL Duax, B. C. Pressman, G. Phillies, B. Tokar, andR. Sanches. Thiswork was supported by an NEI