COLLAGEN-SYNTHESIS IN NORMAL AND OSTEOARTHRITIC HUMAN CARTILAGE
COLLAGEN-SYNTHESIS IN NORMAL AND OSTEOARTHRITIC HUMAN CARTILAGE
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DOI：
10.1172/jci108676
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发表时间：
1977-01-01
影响因子：
15.9
通讯作者：
MANKIN, HJ
中科院分区：
文献类型：
--
作者：
LIPPIELLO, L;HALL, D;MANKIN, HJ
骨关节炎的人关节软骨中的胶原代谢与正常软骨中的胶原代谢进行了比较，并且还与由组织学组织化学分级系统确定的骨关节炎病变的严重程度相关。DNA、羟脯氨酸和羟赖氨酸的浓度与骨关节炎病变的严重程度之间无明显相关性（晚期病变除外）。同样，正常组与骨关节炎组的组织中这些组分的水平也没有相关性。骨关节炎组织中的羟赖氨酸/羟脯氨酸比值与正常组织相比具有相似性，并且随着疾病过程的严重程度增加，这些比值没有变化，这反对骨关节炎与II型胶原合成向I型胶原合成的重大转变相关的可能性。与正常软骨相比，骨关节炎软骨中的[3 H]脯氨酸掺入增加了4倍，并随组织学组织化学分级的进展而变化。测量软骨基质的不溶性含羟脯氨酸材料的比活性，作为胶原蛋白周转的指标，显示骨关节炎软骨增加6倍，这也随级别而变化。这些组织中的胶原合成显著大于非骨关节炎组织中的胶原合成，并且在一定程度上随疾病过程的严重程度而直接变化，然后随着病变变得更严重而反向变化。
Collagen metabolism in osteoarthritic human articular cartilage was compared to that in normal cartilage and was also correlated with the degree of severity of the osteotharitic lesion as determined by a histological-histochemical grading systems. No correlation was apparent between the concentrations of DNA, hydroxyproline and hydroxylysine and the degree of severity of the osteoarthritic lesion (except in far-advanced lesions). Similarly, there was no correlation in levels of these components in tissues from the normal vs. osteoarthritic group. The similarity of the values of the ratio hydroxylysine/hydroxyproline in osteoarthritic tissue compared with normal, and the lack of variation in these with increasing severity of the disease process argues against the possibility that osteoarthritis is associated with a major shift in the synthesis of type II collagen to type I. [3H]Proline incorporation into osteoarthritic cartilage was increased 4-fold as compared to normal cartilage and varied with advancing histological-histochemical grade. Measurement of the specific activity of insolubilized hydroxyproline-containing material of the cartilage matrix, as an index of the turnover of collagen, showed a 6-fold increase in osteoarthritic cartilage which also varied with grade. Collagen synthesis in these tissues is substantially greater than in nonosteoarthritic tissues and varies directly with the severity of the disease process, up to a point, and then varies inversely as the lesion becomes more severe.