HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR HYDROXYCHLOROQUINE AND METABOLITES IN BLOOD AND PLASMA, USING A STATIONARY PHASE OF POLY(STYRENE DIVINYLBENZENE) AND A MOBILE PHASE AT PH 11, WITH FLUORIMETRIC DETECTION
HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR HYDROXYCHLOROQUINE AND METABOLITES IN BLOOD AND PLASMA, USING A STATIONARY PHASE OF POLY(STYRENE DIVINYLBENZENE) AND A MOBILE PHASE AT PH 11, WITH FLUORIMETRIC DETECTION
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DOI：
10.1016/s0378-4347(00)82024-1
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
BROWN, KF
中科院分区：
文献类型：
--
作者：
TETT, SE;CUTLER, DJ;BROWN, KF
建立了一种快速测定羟氯喹及其主要代谢产物去乙基羟氯喹和双去乙基羟氯喹的高效液相色谱法。使用大孔聚合物、聚（苯乙烯二乙烯基苯）、固定相和具有三乙胺的甲醇-水（pH 11）的移动的相的离子抑制分离技术。使用碱化血浆或全血的单次乙醚提取物，加入氯喹作为内标。采用荧光检测，在337 nm处激发，灵敏度为1 ng/ml。一些可同时用于类风湿性疾病的抗炎药物显示不干扰测定。从类风湿患者获得的全血和血浆样品含有羟氯喹和所有三种代谢产物。
A sensitive rapid high-performance liquid chromatographic assay for hydroxychloroquine and three major metabolites, desethylhydroxychloroquine and bisdesethylchloroquine, has been developed. An ion-suppression separation technique is used with a macroporous polymer, poly(styrene divinylbenzene), stationary phase and a mobile phase of methanol-water with triethylamine, pH 11. A single ether extraction from alkalinized plasma or whole blood, with chloroquine added as an internal standard, is used. Using fluorescence detection, with excitation at 337 nm, the limit of sensitivity is 1 ng/ml. Some anti-inflammatory drugs which may be used concurrently for rheumatoid disorders are shown not to interfere with the assay. Samples of whole blood and plasma obtained from rheumatoid patients contained hydroxychloroquine and all three metabolites.