Glucose kinetics in interstitial fluid can be predicted by compartmental modeling.
Glucose kinetics in interstitial fluid can be predicted by compartmental modeling.
复制标题
间质液中的葡萄糖动力学可以通过房室模型来预测。
DOI：
10.1152/ajpendo.1997.272.3.e494
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Wolfe,RR
中科院分区：
文献类型：
--
作者：
Gastaldelli,A;Schwarz,JM;Caveggion,E;Traber,LD;Traber,DL;Rosenblatt,J;Toffolo,G;Cobelli,C;Wolfe,RR
本研究的目的是在绵羊中定义基础条件下葡萄糖动力学的房室模型，并检验通过采样胸导管淋巴液（TDL）获得的间质液代表葡萄糖模型的一个或多个外周房室的假设。在9只动物中注射[6，6-（2）H]葡萄糖推注，随后频繁采集血液和TDL。线性动力学建模已应用于血浆数据，表明三室模型充分描述了葡萄糖动力学。悬链线和乳头状模型进行了鉴定，并在不可访问的池中的示踪剂行为的预测进行了评估。在所有实验中，无论模型结构如何，在与血液交换最快的隔室（池2）中的预测与TDL中测得的示踪剂与示踪剂比率很好地匹配。此外，模拟分析表明，TDL中90-95%的示踪剂来自该池。这支持生理鉴定外周池葡萄糖动力学细胞外液TDL测量所代表的。
The purposes of this study were to define in sheep a compartmental model for glucose kinetics in the basal condition and to test the hypothesis that interstitial fluid obtained by sampling thoracic duct lymph (TDL) represents one or more peripheral compartments of the glucose model. A bolus of [6,6-(2)H]glucose was injected in nine animals, followed by frequent sampling of blood and TDL. Linear kinetic modeling has been applied to plasma data, indicating that a three-compartment model adequately describes glucose kinetics. Both catenary and mammillary models were identified, and their predictions for the tracer behavior in nonaccessible pools were evaluated. In all experiments, regardless of the model structure, predictions in the compartment most rapidly exchanging with blood (pool 2) well matched the measured tracer-to-tracee ratio in TDL. Furthermore, modeling analysis showed that 90-95% of the tracer in TDL comes from this pool. This supported the physiological identification of a peripheral pool of glucose kinetics as extracellular fluid represented by TDL measurements.