PROTHYMOSIN-ALPHA AND PARATHYMOSIN - MESSENGER-RNA AND POLYPEPTIDE LEVELS IN RODENT TISSUES
PROTHYMOSIN-ALPHA AND PARATHYMOSIN - MESSENGER-RNA AND POLYPEPTIDE LEVELS IN RODENT TISSUES
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DOI：
10.1016/0003-9861(89)90107-0
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发表时间：
1989-02-15
影响因子：
3.9
通讯作者：
HORECKER, BL
中科院分区：
文献类型：
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作者：
CLINTON, M;FRANGOULAZARIDIS, M;HORECKER, BL
印迹杂交分析已确定胸腺肽原.α mRNA 的存在。 (ProT.alpha.) 和大鼠和小鼠肺、肝、肾和脑中的甲状胸腺素 (ParaT)，证实了这些肽在非淋巴组织中的生物合成。在这些组织中，mRNA 的水平与多肽的含量平行，这是用特定的放射免疫测定法测定的。 mRNA水平也证实了两种多肽之间的相互关系； ProT.α。其mRNA在脾和胸腺中浓度最高，其次是肺、肾和脑，肝脏中浓度最低。另一方面，肝脏中含有最高浓度的 ParaT 和 ParaT mRNA，而脾脏和胸腺中的水平最低。与年轻（6-8 周）小鼠的组织相比，年长（18 个月）小鼠的两种多肽浓度较低（20-40%），mRNA 含量的下降在性质上相似。
Blot hybridization analyses have established the presence of mRNAs for prothymosin .alpha. (ProT.alpha.) and for parathymosin (ParaT) in rat and mouse lung, liver, kidney, and brain, confirming the biosynthesis of these peptides in nonlymphoid tissues. In these tissues the levels of mRNAs paralleled the content of the polypeptides, determined with specific radioimmunoassays. The mRNA levels also confirmed the reciprocal relation between the two polypeptides; ProT.alpha. and its mRNA were found in highest concentrations in spleen and thymus, followed by lung, kidney, and brain, with lowest concentrations in liver. On the other hand, liver contained highest concentrations of ParaT and the mRNA for ParaT, with lowest levels present in spleen and thymus. In comparison to tissues from young (6-8 week) mice, older (18 month) mice contained lower concentrations (20-40%) of both polypeptides, with qualitatively similar decreases in mRNA content.