Production of Brown/Yellow Patches in the SLC7A11 Transgenic Sheep via Testicular Injection of Transgene
Production of Brown/Yellow Patches in the SLC7A11 Transgenic Sheep via Testicular Injection of Transgene
复制标题
通过睾丸注射转基因在 SLC7A11 转基因羊中产生棕色/黄色斑块
DOI：
10.1016/j.jgg.2012.04.005
复制
发表时间：
2012-06-01
影响因子：
5.9
通讯作者：
Li, Wei
中科院分区：
文献类型：
--
作者：
He, Xin;Li, Hongtao;Li, Wei
据报道，编码溶质载体家族7成员11（阴离子氨基酸转运体轻链，xCT）的基因SLC 7A 11与多种过程有关，如褐黑素产生、细胞增殖和迁移、卡波西肉瘤疱疹病毒（KSHV）进入宿主细胞、学习和记忆。其参与癌细胞增殖和转移已被广泛研究。其在嗜黑质发生中的作用可能在绵羊中是保守的。为研究SLC 7A 11基因在绵羊毛色调控中的作用，从绵羊皮肤成纤维细胞中克隆了SLC 7A 11基因的全长cDNA。绵羊xCT（sxCT）基因的开放阅读框架全长1512 bp，编码503个氨基酸。探讨了其在体外和体内的褐黑素合成中的作用。在主要产生真黑素的melan-a非聚集小鼠黑素细胞中，过表达的sxCT降低了真黑素的含量。使用睾丸注射转基因方法，sxCT转基因绵羊产生，并表现出棕色/黄色的外套补丁，这表明sxCT可以选择性地表达，以增加在羊毛中的褐黑素的生产。我们的研究表明，睾丸注射转基因可用于遗传修饰绵羊毛色。
The gene, SLC7A11, which encodes the solute carrier family 7 member 11 (anionic amino acid transporter light chain, xCT), has been reported to be implicated in multiple processes such as in pheomelanin production, cell proliferation and migration, Kaposi's sarcoma herpesvirus (KSHV) entry into the host cells, learning and memory. Its involvement in cancer cell proliferation and metastasis has been widely studied. Its role in pheomelanogenesis is likely conserved in sheep. The full-length cDNA of sheep SLC7A11 was cloned from sheep skin fibroblasts for evaluating its role in regulating sheep coat color. The complete open reading frame of sheep xCT (sxCT) is 1512 bp in length, encoding a 503 amino acid polypeptide. We explored its function on pheomelanogenesis in vitro and in vivo. In the melan-a non-agouti mouse melanocytes that mainly produce eumelanin, overexpressed sxCT reduced the content of eumelanin. Using a testicular injection transgenic method, sxCT-transgenic sheep were generated and exhibited patches of brown/yellow coat, suggesting that sxCT can be selectively expressed to increase the pheomelanin production in wool. Our studies suggest that testicular injection of transgene can be used to genetically modify sheep coat color.