Nucleotide sequence of avian carcinoma virus MH2: two potential onc genes, one related to avian virus MC29 and the other related to murine sarcoma virus 3611.
Nucleotide sequence of avian carcinoma virus MH2: two potential onc genes, one related to avian virus MC29 and the other related to murine sarcoma virus 3611.
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禽癌病毒MH2的核苷酸序列：两个潜在的onc基因，一个与禽病毒MC29相关，另一个与鼠肉瘤病毒3611相关。
DOI：
10.1073/pnas.81.10.3000
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发表时间：
1984
影响因子：
11.1
通讯作者：
Papas,TS
中科院分区：
文献类型：
--
作者：
Kan,NC;Flordellis,CS;Mark,GE;Duesberg,PH;Papas,TS
禽癌病毒MH 2的5.2-腺苷酸酶（kb）RNA基因组具有遗传结构5'-delta gag（0.2kb）-mht（1.2kb）-myc（1.4kb）-c（0.4kb）-poly（A）（0.2kb）-3'。Δ gag是部分逆转录病毒核心蛋白基因，mht和myc是细胞衍生的MH 2特异性序列，c是3 ′-末端逆转录病毒载体序列。为了阐明该病毒的遗传结构，并与其它含mht和myc的致癌病毒以及鸡原myc基因进行比较，我们测定了从delta gag的3 '端到分子克隆的前病毒MH2 DNA的3'端的3.5kb的核苷酸序列。得到以下结果：（i）Δ gag-mht形成具有2682个核苷酸的连续阅读框的杂合基因，其终止于mht 3 '末端附近的终止密码子. mht的3 '969个核苷酸到终止密码子的序列与小鼠肉瘤病毒3611的onc特异性raf序列有80%的同源性（在推导的氨基酸水平上有94%的同源性）。(ii)myc序列的前面是与细胞原myc基因共有的RNA剪接受体位点，超过该位点，它与禽类逆转录病毒MC 29和鸡原myc的myc序列共线至3'-终止密码子和40个非编码核苷酸。因此，myc与5 '逆转录病毒外显子一起形成第二个MH2特异性基因。(iii)myc后面是约400个核苷酸的3 ′-末端c区，除了长末端重复序列的5 ′末端附近的一个取代外，它与Rous肉瘤病毒的c区共线。结论MH2含有两个具有致癌潜力的基因，即与MSV 3611的Δ gag-raf转化基因密切相关的Δ gag-mht基因和与MC 29的转化基因相关的myc基因。此外，可以得出结论，细胞原onc基因，其在序列转导后成为病毒onc基因，是一个小的组，因为在19个已知onc序列中，5个被不同的病毒分类组共享，其中mht/raf同源性是迄今为止确定的最接近的。
The 5.2-kilobase (kb) RNA genome of avian carcinoma virus MH2 has the genetic structure 5'-delta gag (0.2 kb)- mht (1.2 kb)-myc (1.4 kb)-c (0.4 kb)-poly(A) (0.2 kb)-3'. delta gag is a partial retroviral core protein gene, mht and myc are cell-derived MH2-specific sequences, and c is the 3'-terminal retroviral vector sequence. Here we have determined the nucleotide sequence of 3.5 kb from the 3' end of delta gag to the 3' end of molecularly cloned proviral MH2 DNA, in order to elucidate the genetic structure of the virus and to compare it with other mht - and myc-containing oncogenic viruses as well as with the chicken proto-myc gene. The following results were obtained: (i) delta gag- mht forms a hybrid gene with a contiguous reading frame of 2682 nucleotides that terminates with a stop codon near the 3' end of mht . The 3' 969 nucleotides of mht up to the stop codon are 80% sequence related to the onc-specific raf sequence of murine sarcoma virus 3611 (94% homologous at the deduced amino acid level). (ii) The myc sequence is preceded by an RNA splice acceptor site shared with the cellular proto-myc gene, beyond which it is colinear up to a 3'-termination codon and 40 noncoding nucleotides with the myc sequences of avian retrovirus MC29 and chicken proto-myc. Thus, myc forms, together with a 5' retroviral exon, a second MH2-specific gene. (iii) myc is followed by the 3'-terminal c region of about 400 nucleotides, which is colinear with that of Rous sarcoma virus except for a substitution near the 5' end of the long terminal repeat. It is concluded that MH2 contains two genes with oncogenic potential, the delta gag- mht gene, which is closely related to the delta gag-raf transforming gene of MSV 3611, and the myc gene, which is related to the transforming gene of MC29. Furthermore, it may be concluded that the cellular proto-onc genes, which on sequence transduction become viral onc genes, are a small group because among the 19 known onc sequences, 5 are shared by different taxonomic groups of viruses of which the mht /raf homology is the closest determined so far.