Oxidative Enzymes of the Interalveolar Septum of the Rat 1
Oxidative Enzymes of the Interalveolar Septum of the Rat 1
复制标题
大鼠肺泡间隔氧化酶1
作者：
W. Tyler;A. Pearse
虽然肺泡间隔是许多有害物质的靶组织，并且其组成细胞类型之一被认为是正常肺功能所必需的表面活性剂的来源（Klaus，Reiss，Tooley，Piel和Clements，1962），但关于该组织的代谢潜力知之甚少。组织化学方法，虽然主要是定性的，允许精确定位的化学活动之间的各种组成部分的一个器官。这种类型的信息是不能通过经典的生物化学方法提取，分馏或组织孵育。本研究的目的是揭示肺泡隔细胞的代谢潜力。已报道的少数组织化学研究仅涉及少数组织化学上可证实的氧化酶（Pliess和苏尔，1959; Sorokin、Padykula和赫尔曼，1959; Sorokin，1961）。研究数量较少，部分原因似乎是难以制备适合脱氢酶组织化学的肺切片。作者设计了一种简单的方法制备适用于组织化学方法的膨胀肺冰冻切片。
Though the interalveolar septum is the target tissue for many deleterious agents, and one of its constituent cell-types is thought to be the source of a surfactant essential to normal pulmonary function (Klaus, Reiss, Tooley, Piel, and Clements, 1962), little is known concerning the metabolic potential of this tissue. Histochemical methods, though mainly qualitative, permit precise localization of chemical activities among various components of an organ. This type of information is not obtainable by the classical biochemical methods of extraction, fractionation or tissue incubation. The object of this study is to reveal the metabolic potential of the cells of the interalveolar septum. The few histochemical studies that have been reported have concerned only a few of the histochemically demonstrable oxidative enzymes (Pliess and Suhr, 1959; Sorokin, Padykula, and Herman, 1959; Sorokin, 1961). The small number of studies appears to be due in part to the difficulty in preparing sections of lung suitable for dehydrogenase histochemistry. The authors have devised a simple method for preparing thin cryostat sections of distended lung which are suitable for histochemical procedures.