Analysis of chromatin remodeling during formation of a DNA double-strand break at the yeast mating type locus.
Analysis of chromatin remodeling during formation of a DNA double-strand break at the yeast mating type locus.
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酵母交配型基因座 DNA 双链断裂形成过程中染色质重塑的分析。
DOI：
10.1016/j.ymeth.2009.02.007
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Osley,MaryAnn
中科院分区：
文献类型：
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作者：
Tsukuda,Toyoko;Trujillo,KellyM;Martini,Emmanuelle;Osley,MaryAnn
DNA修复发生在染色质背景下，核小体重塑现在被认为是一个重要的调节功能，允许修复因子进入受损部位。酵母交配型基因座（MAT）已成为研究DNA双链断裂（DSB）时染色质重塑的一个很好的模型。我们讨论的方法来研究核小体动力学和DSB修复因子招聘MAT位点后，DSB已经形成。
DNA repair occurs in a chromatin context, and nucleosome remodeling is now recognized as an important regulatory feature by allowing repair factors access to damaged sites. The yeast mating type locus (MAT) has emerged an excellent model to study the role of chromatin remodeling at a well-defined DNA double-strand break (DSB). We discuss methods to study nucleosome dynamics and DSB repair factor recruitment to the MAT locus after a DSB has been formed.