Are allergens more abundant and/or more stable than other proteins in pollens and dust?
Are allergens more abundant and/or more stable than other proteins in pollens and dust?
复制标题
DOI：
10.1111/all.14121
复制
发表时间：
2019-12-10
期刊：
影响因子：
12.4
通讯作者：
Mueller, Geoffrey A.
中科院分区：
文献类型：
--
作者：
Cabrera, Aurora;Randall, Thomas A.;Mueller, Geoffrey A.
The human exposome contains millions of proteins, yet only a select few consistently become allergens. This has led to speculation that there are intrinsic factors which differentiate allergens from non-allergens. Anecdotal evidence suggested that abundance and stability could be two such properties, but no rigorous statistical comparisons were available until a study of the dust mite Dermatophagoides pteronyssinus (Dp) combined transcriptomics with proteomics (1). The Dp study measured transcription levels as a proxy for protein abundance and correlated the results with proteome-wide stability measurements using mass spectrometry. It was found that the mite allergens were as a population more stable and more highly expressed than Dp non-allergens. Dp was a good model system to study due to its clinical relevance and its large number of characterized allergens, providing statistical power for the conclusions. However, the broader applicability of this correlation to other sources of allergens remained unproven.The current study seeks to address this question through applying similar analyses to a range of indoor and outdoor allergen sources. Pollen extracts of the important allergen sources of grass Phleum pratense (Pp), ragweed Ambrosia artemisiifolia (Aa), and birch tree Betula pendula (Bv) were prepared from purified pollen to measure protein stabilities as previously described (1). An extract of cockroach Blatella germanica (Bg) frass was kindly provided by Dr. Anna Pomés (Indoor Biotechnologies) and similarly analyzed to measure protein stabilities. Frass is the debris associated with cockroaches in captivity including fecal particles and body parts, which was judged to better represent human exposure as opposed to whole cockroach extract. Additionally, Bg whole extracts tend to be treated with acetone as part of the delipidation process, potentially altering protein content and/or stability. Transcription data was assembled and analyzed from publicly available data sets
