Draft Genome Sequence of Streptomyces clavuligerus NRRL 3585, a Producer of Diverse Secondary Metabolites
Draft Genome Sequence of Streptomyces clavuligerus NRRL 3585, a Producer of Diverse Secondary Metabolites
复制标题
DOI：
10.1128/jb.00859-10
复制
发表时间：
2010-12-01
影响因子：
3.2
通讯作者：
Kim, Jihyun F.
中科院分区：
文献类型：
--
作者：
Song, Ju Yeon;Jeong, Haeyoung;Kim, Jihyun F.
棒状链霉菌是一种重要的工业菌株，能产生克拉维酸和头霉素C等多种抗生素。高质量的S.通过采用包括桑格测序、Roche/454焦磷酸测序、光学作图和部分精加工的杂交方法来产生clavuligerus NRRL 3585菌株。它的基因组由四个线性复制子、一条染色体和四个质粒组成，携带着参与次级代谢产物（包括多种抗生素）生物合成的多组基因。
Streptomyces clavuligerus is an important industrial strain that produces a number of antibiotics, including clavulanic acid and cephamycin C. A high-quality draft genome sequence of the S. clavuligerus NRRL 3585 strain was produced by employing a hybrid approach that involved Sanger sequencing, Roche/454 pyrosequencing, optical mapping, and partial finishing. Its genome, comprising four linear replicons, one chromosome, and four plasmids, carries numerous sets of genes involved in the biosynthesis of secondary metabolites, including a variety of antibiotics.