Molecular cloning of the bovine prolactin receptor and distribution of prolactin and growth hormone receptor transcripts in fetal and utero-placental tissues.
Molecular cloning of the bovine prolactin receptor and distribution of prolactin and growth hormone receptor transcripts in fetal and utero-placental tissues.
复制标题
牛催乳素受体的分子克隆以及催乳素和生长激素受体转录本在胎儿和子宫胎盘组织中的分布。
DOI：
10.1016/0303-7207(92)90210-w
复制
发表时间：
1992
影响因子：
4.1
通讯作者：
Schuler,LA
中科院分区：
文献类型：
--
作者：
Scott,P;Kessler,MA;Schuler,LA
我们从子宫内膜的cDNA文库中克隆了一个牛催乳素受体(BPRL)的cDNA，它预测了一个557个氨基酸的跨膜蛋白，类似于其他特征的催乳素受体的长形式。预测的细胞质结构域被人类肝脏转录本中使用的终止密码子略微截断，该终止密码子位于36个密码子5‘。在COS细胞中表达时，编码一个与牛胎盘催乳素结合的蛋白，其亲和力接近(牛胎盘催乳素的KD为2.0 3×10−10M；牛胎盘催乳素的KD为3.0 7×10−10M)。Northern分析显示有多个转录本，其中母体肝脏、黄体、肠、子宫内膜和胎肝含有一个约3.8kb的主要转录本，母体黄体和子宫内膜是第二大小的转录本，其丰度似乎相等，为4.4kb。这种差异似乎不在编码区内。对母体肝脏和子宫内膜转录本进行的引物延伸分析显示了相当大的异质性。用半定量逆转录聚合酶链式反应检测孕中期催乳素和生长激素受体转录本的分布，发现两者都广泛存在于牛胎儿和胎盘组织中。牛催乳素受体基因的分离和受体分布的描述将有助于研究该基因家族的胎盘和脑垂体成员在妊娠期间的作用。
We have isolated a bovine prolactin (bPRL) receptor cDNA from an endometrial cDNA library, which predicts a 557 amino acid transmembrane protein similar to the long forms of other characterized prolactin receptors. The predicted cytoplasmic domain is slightly truncated primarily by a stop codon located 36 codons 5'from the stop utilized in the human hepatic transcript. When expressed in COS cells, this cDNA was shown to encode a protein which bound bovine placental lactogen (bPL) and bPRL with nearly equal affinity (K D for bPL, 2.03× 10− 10 M; bPRL, 3.07× 10− 10 M). Northern analysis demonstrated multiple transcripts, with maternal liver, corpus luteum, intestine, endometrium and fetal liver containing a major transcript of about 3.8 kb, and maternal corpus luteum and endometrium, a second sized transcript of apparently equal abundance of 4.4 kb. This difference did not appear to be within the coding region. Primer extension analysis of maternal hepatic and endometrial transcripts revealed considerable heterogeneity. Examination of the distribution of prolactin and growth hormone receptor transcripts at mid-pregnancy by semi-quantitative reverse transcriptase polymerase chain reaction showed that both are widespread in bovine fetal and placental tissues. This isolation of bovine prolactin receptor cDNA, and description of receptor distribution will facilitate study of the action of the placental and pituitary members of this gene family during pregnancy.