MURINE DENDRITIC CELLS PULSED IN-VITRO WITH TUMOR-ANTIGEN INDUCE TUMOR RESISTANCE IN-VIVO
MURINE DENDRITIC CELLS PULSED IN-VITRO WITH TUMOR-ANTIGEN INDUCE TUMOR RESISTANCE IN-VIVO
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DOI：
10.1002/eji.1830240317
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发表时间：
1994-03-01
影响因子：
5.4
通讯作者：
MOSER, M
中科院分区：
文献类型：
--
作者：
FLAMAND, V;SORNASSE, T;MOSER, M
这项工作的目的是利用免疫系统的元件在 BALB/c 小鼠中诱导肿瘤对 B 细胞淋巴瘤的抵抗。我们和其他人确实已经证明，像树突状细胞这样的抗原呈递细胞（APC）可以诱导有效的免疫反应，甚至可以替代弗氏佐剂。在这里，我们表明，用肿瘤抗原（淋巴瘤细胞表达的 BCL1 独特型）体外脉冲的同基因树突状细胞免疫的小鼠可以免受随后的肿瘤接种的影响。体内抗性可以与对肿瘤表达的独特型特异的体液反应的诱导相关。当B细胞用作APC时，无法实现这种保护。这些数据表明，可以使用树突状细胞来招募和激活荷瘤动物中的效应细胞，从而提供持久的免疫监视。
The aim of this work is to induce tumor resistance to a B cell lymphoma in BALB/c mice using elements of the immune system. It has indeed been shown by us and by others that antigen-presenting cells (APC) like dendritic cells can induce efficient immune responses and can even substitute for Freund's adjuvant. Here we show that mice immunized with syngeneic dendritic cells pulsed in vitro with tumor antigen (BCL1 idiotype expressed by lymphoma cells) are protected against a subsequent tumor inoculation. The in vivo resistance can be correlated with the induction of a humoral response specific for the idiotype expressed by the tumor. No such protection can be achieved when B cells are used as APC. These data show that effector cells in tumor-bearing animals can be recruited and activated using dendritic cells, providing long-lasting immune surveillance.