CHROMOSOMAL PROGRESSION OF MALIGNANT HUMAN GLIOMAS FROM BIOPSY TO ESTABLISHMENT AS PERMANENT LINES INVITRO
CHROMOSOMAL PROGRESSION OF MALIGNANT HUMAN GLIOMAS FROM BIOPSY TO ESTABLISHMENT AS PERMANENT LINES INVITRO
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DOI：
10.1016/0165-4608(87)90094-x
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发表时间：
1987-01-01
影响因子：
--
通讯作者：
BIGNER, DD
中科院分区：
文献类型：
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作者：
BIGNER, SH;MARK, J;BIGNER, DD
从直接制备和/或短期培养到体外建立，对四种恶性胶质瘤的核型进行了跟踪，以确定培养细胞是否保持原始核型，还是体外选择、进展或改变的产物。这四种人类胶质瘤衍生细胞系的核型表现出相同的进化模式，包括干细胞加倍或密切相关的群体；其中一个品系再次改变倍性为近五倍体。最初看到的标记类型通常被保留，但在后来的传代中获得了新的标记。我们得出结论，这四种人类胶质瘤衍生细胞系的最终染色体组成是核型进化的产物，而不是一小部分多倍体细胞的简单选择。
Karyotypes of four malignant human gliomas were followed from direct preparation and/or short-term culture through their establishment in vitro to determine whether the cultured cells maintained the original karyotypes, or were the products of selection, progression, or alteration in vitro. The karyotypes of these four human glioma-derived cell lines showed the same evolutionary pattern consisting of a doubling of the stem line or a closely related population; one line changed ploidy again to near-pentaploid. Marker types seen originally were generally retained, but new markers were acquired in the later passages. We concluded that the eventual chromosomal compositions of these four human glioma-derived cell lines were the products of karyptypic evolution, rather than simple selection of a minor population of polyploid cells.