Advances in nonlinear optical microscopy for visualizing dynamic tissue properties in culture.
Advances in nonlinear optical microscopy for visualizing dynamic tissue properties in culture.
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DOI：
10.1089/teb.2007.0284
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发表时间：
2008-03
期刊：
影响因子：
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通讯作者：
A. Yeh;H. Gibbs;Jin-Jia Hu;Adam M. Larson
中科院分区：
文献类型：
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作者：
A. Yeh;H. Gibbs;Jin-Jia Hu;Adam M. Larson
光学显微镜包括高分辨率成像技术，可用于非破坏性地研究和表征活的生物系统和培养中的工程组织构建体。特别是，非线性光学显微镜（NLOM）是非常适合的可视化和量化的过程中涉及的细胞-细胞外基质的相互作用在体内。目前的NLOM技术能够实现伴随的分子成像和微观结构组织的可视化，其可以在微观长度尺度上提供信号转导和生物效应之间的直接联系，最终形成组织的宏观性质和功能。这篇综述强调了光与组织之间的非线性光学相互作用的基本原理，并提出了未来技术发展的方向，以更好地补充现代生命科学的定量，高通量分析。
Optical microscopy encompasses high-resolution imaging techniques that can be used to non-destructively investigate and characterize living biological systems and engineered tissue constructs in culture. In particular, nonlinear optical microscopy (NLOM) is well suited for the visualization and quantification of processes involved in cell-extracellular matrix interactions in vivo. Current NLOM technology enables concomitant molecular imaging and visualization of microstructural organization that could provide a direct link between signal transduction and biological effect at microscopic length scales that culminate into tissue macroscopic properties and function. This review highlights the fundamentals of nonlinear optical interactions between light and tissue and presents a direction for future technology development to better complement quantitative, high-throughput assays of the modern life sciences.