The Shift of ERG B-Wave Induced by Hours' Dark Exposure in Rodents.
The Shift of ERG B-Wave Induced by Hours' Dark Exposure in Rodents.
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啮齿动物数小时黑暗暴露引起的 ERG B 波变化
DOI：
10.1371/journal.pone.0161010
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Yu H
中科院分区：
文献类型：
--
作者：
Li D;Fang Q;Yu H
黑暗的适应性可以诱导视网膜的快速功能转移，在此之后，视网膜功能在连续的黑暗暴露期间保持稳定。
轻度操纵后的成人和啮齿动物的体内记录。
我们揭示了成年大鼠的ERG B波降低了五倍，而在第一个小时的黑暗暴露中，ERG B波显着增加，但是在1天后，一日曝光后，持续的曝光效果是完全回收的。在非暴露的眼睛中，无法恢复延长的黑暗暴露诱导的功能转移，在强度响应曲线的放大器上的下调中，响应光强度的动态范围在很大程度上是稳定的。 ERG A波的典范没有长时间的黑暗暴露后发生了重大变化。
这种不依赖年龄的ERG变化表明，视网膜的一般功能转移，这是在视觉系统的入门级别。
Purpose Dark adaptation can induce a rapid functional shift in the retina, and after that, the retinal function is believed to remain stable during the continuous dark exposure. However, we found that electroretinograms (ERG) b-waves gradually shifted during 24 hours’ dark exposure in rodents. Detailed experiments were designed to explore this non-classical dark adaptation. Methods In vivo ERG recording in adult and developing rodents after light manipulations. Results We revealed a five-fold decrease in ERG b-waves in adult rats that were dark exposed for 24 hours. The ERG b-waves significantly increased within the first hour’s dark exposure, but after that decreased continuously and finally attained steady state after 1 day’s dark exposure. After 3 repetitive, 10 minutes’ light exposure, the dark exposed rats fully recovered. This recovery effect was eye-specific, and light exposure to one eye could not restore the ERGs in the non-exposed eye. The prolonged dark exposure-induced functional shift was also reflected in the down-regulation on the amplitude of intensity-ERG response curve, but the dynamic range of the responsive light intensity remained largely stable. Furthermore, the ERG b-wave shifts occurred in and beyond classical critical period, and in both rats and mice. Importantly, when ERG b-wave greatly shifted, the amplitude of ERG a-wave did not change significantly after the prolonged dark exposure. Conclusions This rapid age-independent ERG change demonstrates a generally existing functional shift in the retina, which is at the entry level of visual system.