Molecular response to surgical stress: specific and simultaneous heat shock protein induction in the adrenal cortex, aorta, and vena cava.
Molecular response to surgical stress: specific and simultaneous heat shock protein induction in the adrenal cortex, aorta, and vena cava.
复制标题
对手术应激的分子反应：肾上腺皮质、主动脉和腔静脉中特异性且同时的热休克蛋白诱导。
作者：
R. Udelsman;M. J. Blake;N. Holbrook
对手术应激的内分泌反应导致下丘脑-垂体-肾上腺（HPA）轴和交感神经系统的激活。细胞对各种应激的反应导致称为热休克蛋白的应激反应蛋白家族的合成。尚未在体内研究内分泌和细胞应激反应之间的潜在相互作用。建立了一个手术模型来确定对手术应激的遗传反应。Wistar大鼠进行乙醚麻醉，剖腹手术，出血，和变量恢复期。随后收获组织，分离RNA并探测HSP 70信使RNA水平。这些研究表明，HSP 70的强烈诱导，但仅在肾上腺，主动脉和腔静脉。这种特异性诱导是快速的，发生在手术后30分钟，并且是显著的（大于20倍诱导）。诱导发生在平行HPA轴激活和肾上腺皮质特异性通过原位杂交确定。这些观察结果表明，分子应激反应和HPA轴激活之间的功能相互作用。
The endocrine response to surgical stress results in activation of the hypothalamic-pituitary-adrenal (HPA) axis and the sympathetic nervous system. The cellular response to a wide variety of stresses results in the synthesis of a family of stress response proteins termed heat shock proteins. Potential interactions between endocrine and cellular stress responses have not been investigated in vivo. A surgical model was developed to define the genetic response to surgical stress. Wistar rats underwent ether anesthesia, laparotomy, hemorrhage, and variable recovery periods. Tissues were subsequently harvested and the RNA was isolated and probed for HSP70 messenger RNA levels. These studies showed a strong induction of HSP70 but only in the adrenal gland, aorta, and vena cava. This specific induction was rapid, occurring 30 minutes after surgery, and dramatic (greater than twentyfold induction). The induction occurred in parallel with HPA axis activation and was adrenal cortical specific as determined by in situ hybridization. These observations suggest a functional interaction between the molecular stress response and HPA axis activation.