Human hamstring tenocytes survive when seeded into a decellularized porcine Achilles tendon extracellular matrix
Human hamstring tenocytes survive when seeded into a decellularized porcine Achilles tendon extracellular matrix
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DOI：
10.3109/03008207.2013.820283
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发表时间：
2013-01-01
影响因子：
2.9
通讯作者：
Schulze-Tanzil, Gundula
中科院分区：
文献类型：
--
作者：
Lohan, Anke;Stoll, Christiane;Schulze-Tanzil, Gundula
肌腱断裂和缺损仍然是骨科的主要挑战。肌腱愈合是一个耗时的过程，导致疤痕组织的生物力学能力改变。利用异种肌腱细胞外基质（ECM）作为天然支架，可将自体人肌腱细胞重新植入，可能是重建受损肌腱的一种很有前途的方法。为此，将猪跟腱作为支架与人细胞供体肌腱进行了组织学表征。采用细胞离心、旋转培养和细胞注射技术，对AS肌腱进行脱细胞处理，再植入人腘绳肌原代肌腱细胞。通过活力测试、组织学和糖胺聚糖/DNA定量来证明肌腱再植的成功。猪AS肌腱的细胞和硫酸氨基糖聚糖含量高于人细胞供体肌腱。完全脱细胞是可以实现的，但会导致硫代糖胺聚糖的洗涤。然而，猪肌腱可以用重要的人腱细胞再细胞化。与天然肌腱相比，再细胞化导致细胞数量的轻微增加和一些糖胺聚糖的恢复。本研究表明，猪肌腱可以用成人肌腱细胞去细胞化和再细胞化。未来的工作应该优化再细胞化肌腱ECM内的细胞分布，并考虑肌腱和供体物种依赖的差异。
Tendon ruptures and defects remain major orthopaedic challenges. Tendon healing is a time-consuming process, which results in scar tissue with an altered biomechanical competence. Using a xenogeneic tendon extracellular matrix (ECM) as a natural scaffold, which can be reseeded with autologous human tenocytes, might be a promising approach to reconstruct damaged tendons. For this purpose, the porcine Achilles (AS) tendons serving as a scaffold were histologically characterized in comparison to human cell donor tendons. AS tendons were decellularized and then reseeded with primary human hamstring tenocytes using cell centrifuging, rotating culture and cell injection techniques. Vitality testing, histology and glycosaminoglycan/DNA quantifications were performed to document the success of tendon reseeding. Porcine AS tendons were characterized by a higher cell and sulfated glycosaminoglycan content than human cell donor tendons. Complete decellularization could be achieved, but led to a wash out of sulfated glycosaminoglycans. Nevertheless, porcine tendon could be recellularized with vital human tenocytes. The recellularization led to a slight increase in cell number compared to the native tendon and some glycosaminoglycan recovery. This study indicates that porcine tendon can be de-and recellularized using adult human tenocytes. Future work should optimize cell distribution within the recellularized tendon ECM and consider tendon-and donor species-dependent differences.