A note on the identity of porcine liver carboxylesterase and prolyl-beta-naphthylamidase.
A note on the identity of porcine liver carboxylesterase and prolyl-beta-naphthylamidase.
复制标题
关于猪肝羧酸酯酶和脯氨酰-β-萘酰胺酶的鉴定的注释。
DOI：
10.1515/bchm3.1993.374.7-12.1033
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
K. Peter
中科院分区：
文献类型：
--
作者：
E. Heymann;K. Peter
通过等电聚焦实验和苯甲基磺酰氟抑制研究，比较了猪肝中的氨基-β-萘酰胺酶和两种流行的猪肝羧酸酯酶的同工酶。结果表明，该酶与羧酸酯酶的酰胺裂解同工酶相同，而与通常占主导地位的丁酸甲酯水解酶不同。最近发表的脯氨酰β-萘酰胺酶序列是否属于这种酶，或者属于优势的没有酰胺酶活性的羧酸酯酶，这是值得怀疑的。令人惊讶的是，大鼠肝脏中裂解酰胺的羧酸酯酶同工酶几乎不具有脯氨基-β-萘酰胺的活性。
Prolyl-beta-naphthylamidase from porcine liver is compared with the two prevalent isoenzymes of pig liver carboxylesterase by isoelectrofocusing experiments and by inhibition studies with phenyl-methyl-sulfonyl fluoride. The results suggest that prolyl-beta-naphthylamidase is identical with the amide-cleaving isoenzyme of carboxylesterase, not with the usually predominant methyl butyrate-hydrolysing isoenzyme. It is questionable whether the recently published sequence of prolyl-beta-naphthylamidase does belong to this enzyme or to the predominant carboxylesterase without amidase activity. Surprisingly, the amide-cleaving carboxylesterase isoenzymes from rat liver have almost no activity with prolyl-beta-naphthylamide.