Pre-staining paper chromatography method for quantification of γ-aminobutyric acid
Pre-staining paper chromatography method for quantification of γ-aminobutyric acid
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DOI：
10.1016/j.chroma.2009.04.044
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发表时间：
2009-06-19
影响因子：
4.1
通讯作者：
Huang, Zhibing
中科院分区：
文献类型：
--
作者：
Li, Haixing;Qiu, Ting;Huang, Zhibing
常规的纸色谱法包括五个步骤：斑点、分离、干燥、喷涂/浸泡和显色。本文建立了一种仅由斑点、分离和显色组成的预染色纸色谱法，用于γ -氨基丁酸的定量分析。与常规纸色谱法相比，该方法具有清洁、快速、成本低、重复性好等优点。优化了茚三酮浓度、显色温度、显色时间和Cu2+浓度对茚三酮反应显色率的影响。采用预染色纸层析-可见分光光度法对-氨基丁酸进行定量分析。结果表明，该方法的检出限为0.05 mg mL(-1)，线性范围为0.5 ~ 20.0 mg mL（-1）。相关系数R-2 = 0.998。方法准确（RSD）
The routine method of paper chromatography includes five steps: spotting, separating, drying, spraying/immersing and color development. In this paper, a pre-staining paper chromatography which only consisted of spotting, separating and color development was developed for quantitative analysis of gamma-aminobutyric acid. Compared to the routine paper chromatography, the improved method is clean, rapid, inexpensive and reproducible. The effects of ninhydrin concentration, color temperature, color time and Cu2+ concentration on the color yield in the ninhydrin reaction were optimized. And then the pre-staining paper chromatography coupled with vis spectrophotometry, was applied to gamma-aminobutyric acid quantification. The results indicated that the limit of detection was 0.05 mg mL(-1) and the linear range was from 0.5 to 20.0 mg mL(-1). Furthermore, an excellent correlation coefficient was observed with an R-2 = 0.998. The method is accurate (RSD