Efficacy of Listerine® Antiseptic in reducing viral contamination of saliva
Efficacy of Listerine® Antiseptic in reducing viral contamination of saliva
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DOI：
10.1111/j.1600-051x.2005.00673.x
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发表时间：
2005-04-01
影响因子：
6.7
通讯作者：
DePaola, LG
中科院分区：
文献类型：
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作者：
Mellier, TF;Silva, A;DePaola, LG
目的：从潜在的临床意义来看，口腔抗菌含漱液的抗病毒效果尚未得到充分的研究。作为一项关于口服防腐剂对1型单纯疱疹病毒的体外研究的后续研究，本研究旨在评估一种含有精油的口服防腐剂在活动性病毒感染期间降低唾液中病毒滴度的体内效果。方法：患者在口周暴发时采用一次性访问方案进行评估，历史和临床上与复发性唇疱疹一致。皮损/口腔液的细胞学涂片直接免疫荧光用于确定单纯疱疹病毒I型或II型。患者被随机分成两组：(1)有效成分组和(2)无菌水对照组。根据标准方案对病毒病变进行临床分期评价。取唾液样本：(1)基线；(2)S含漱30分钟后立即取唾液；(3)30分钟。S冲洗30次后；(4)重复试验，也是60min。S 30岁后用清水冲洗。结果：试验1样本人群中男性19人，女性21人，平均年龄29.2岁；试验2人群中，男性21人，女性19人，平均年龄28岁。在试验1和试验2中，在S 30次实验性冲洗后，可恢复的感染性病毒颗粒减少到零；而对照冲洗导致不显著的减少(P>；0.05)。试验组在30分钟内也表现出持续显著的下降(p<；0.05)。冲洗后与基线相比，而对照组恢复到基线水平。在试验2中，60分钟。漱口后的随访显示，实验组的残留量比基线减少了1-2log残留量；但这并不显著。结论：使用含抗菌剂Listerine(R)防腐剂的口腔含漱剂至少30分钟，在减少口腔液体中病毒污染方面具有临床疗效。在口腔冲洗后。在人与人之间的接触或牙科治疗期间，这些口腔液产生病毒交叉污染的风险可能会降低。
Aim: The anti-viral efficacy of oral antimicrobial rinses has not been adequately studied in terms of potential clinical significance. As a follow-up to an in vitro study on the effect of oral antiseptics on Herpes simplex virus, Type 1, this study was undertaken to evaluate the in vivo effect of an essential oil containing oral antiseptic on the reduction of viral titer in saliva during active viral infection.Method: Patients were recruited and evaluated in a single visit protocol at the onset of a perioral outbreak, consistent historically and clinically with recurrent Herpes labialis. Direct immunofluorescence of cytological smears of the lesions/oral fluids was used to confirm Herpes simplex virus types I or II. Patients were randomly assigned to one of two treatment groups: (1) active ingredient and (2) sterile water control. The viral lesion was evaluated as to clinical stage according to standard protocol. Salivary fluid samples were taken: (1) at baseline; (2) immediately following a 30 s rinse; (3) 30 min. after the 30 s rinse; and (4) on the repeat trial, also at 60 min. after the 30 s rinse. All samples were evaluated for viral titer and results compared.Results: In Trial 1, the sample population consisted of 19 males and 21 females with an average age of 29.2 and in Trial 2, 21 males, 19 females with an average age of 28. In both Trials I and 2, recoverable infectious virions were reduced to zero after a 30 s experimental rinse; whereas, the control rinse resulted in a non-significant (P>0.05) reduction. The experimental group also demonstrated a continued significant (p < 0.05) reduction 30 min. post rinse when compared with baseline while the control group returned to baseline levels. In Trial 2, the 60 min. post rinse follow-up demonstrated a 1-2 log residual reduction from baseline in the experimental group; however, this was not significant.Conclusions: There is clinical efficacy in utilizing an oral rinse with the antimicrobial agent Listerine(R) Antiseptic in reducing the presence of viral contamination in oral fluids for at least 30 min. after oral rinse. The risk of viral cross contamination generated from these oral fluids in person to person contact or during dental treatment may be reduced.