Rhamnose biosynthesis pathway supplies precursors for primary and secondary metabolism in Saccharopolyspora spinosa
Rhamnose biosynthesis pathway supplies precursors for primary and secondary metabolism in Saccharopolyspora spinosa
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DOI：
10.1128/jb.183.19.5632-5638.2001
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发表时间：
2001-10-01
影响因子：
3.2
通讯作者：
Merlo, DJ
中科院分区：
文献类型：
--
作者：
Madduri, K;Waldron, C;Merlo, DJ
鼠李糖是防虫剂多杀菌素的重要成分。然而，刺糖多孢菌参与鼠李糖生物合成的四种酶的编码基因位于基因组的三个不同区域，均与Spinosyn生物合成所需的其他基因簇无关。任何鼠李糖基因的破坏都会导致菌丝体高度碎裂的突变株，这些突变株只能在添加渗透压稳定剂的培养基中存活。这一组基因似乎为细胞壁的合成和次生代谢物的产生提供了鼠李糖。复制该途径的前两个基因可以显著提高Spinosyn发酵产物的产量。
Rhamnose is an essential component of the insect control agent spinosad. However, the genes coding for the four enzymes involved in rhamnose biosynthesis in Saccharopolyspora spinosa are located in three different regions of the genome, all unlinked to the cluster of other genes that are required for spinosyn biosynthesis. Disruption of any of the rhamnose genes resulted in mutants with highly fragmented mycelia that could survive only in media supplemented with an osmotic stabilizer. It appears that this single set of genes provides rhamnose for cell wall synthesis as well as for secondary metabolite production. Duplicating the first two genes of the pathway caused a significant improvement in the yield of spinosyn fermentation products.