Reduced DNA methylation in Arabidopsis thaliana results in abnormal plant development
Reduced DNA methylation in Arabidopsis thaliana results in abnormal plant development
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DOI：
10.1073/pnas.93.16.8449
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发表时间：
1996-08-06
影响因子：
11.1
通讯作者：
Dennis, ES
中科院分区：
文献类型：
--
作者：
Finnegan, EJ;Peacock, WJ;Dennis, ES
用拟南芥甲基转移酶cDNA （METI）的反义结构转化的拟南芥植物减少了CG二核苷酸的胞嘧啶甲基化。5个独立转化子后代的甲基化水平在野生型的10%到100%之间，通过性杂交分离去除反义结构并不能完全恢复后代的甲基化模式，这表明甲基化模式受减数分裂遗传的影响。甲基化降低的植株表现出许多表型和发育异常，包括顶端优势度降低、植株尺寸变小、叶片大小和形状改变、育性下降和开花时间改变，花器官表现出与花同源基因AGAMOUS和APETALA3在叶组织中的异位表达相关的同源转化。这些观察结果表明，DNA甲基化在调节植物的许多发育途径中起着重要作用，甲基转移酶反义植物的发育异常可能是由于基因表达失调引起的。
Arabidopsis plants transformed with an antisense construct of an Arabidopsis methyltransferase cDNA (METI) have reduced cytosine methylation in CG dinucleotides. Methylation levels in progeny of five independent transformants ranged from 10% to 100% of the wild type, Removal of the antisense construct by segregation in sexual crosses did not fully restore methylation patterns in the progeny, indicating that methylation patterns are subject to meiotic inheritance in Arabidopsis. Plants with decreased methylation displayed a number of phenotypic and developmental abnormalities, including reduced apical dominance, smaller plant size, altered leaf size and shape, decreased fertility, and altered flowering time, Floral organs showed homeotic transformations that were associated with ectopic expression of the floral homeotic genes AGAMOUS and APETALA3 in leaf tissue, These observations suggest that DNA methylation plays an important role in regulating many developmental pathways in plants and that the developmental abnormalities seen in the methyltransferase antisense plants may be due to dysregulation of gene expression.