Application of the ADVIA cerebrospinal fluid assay to count residual red blood cells in blood components
Application of the ADVIA cerebrospinal fluid assay to count residual red blood cells in blood components
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DOI：
10.1111/j.1423-0410.2012.01601.x
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发表时间：
2012-10-01
期刊：
影响因子：
2.7
通讯作者：
Devine, D. V.
中科院分区：
文献类型：
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作者：
Culibrk, B.;Stone, E.;Devine, D. V.
背景和目的尽管输注不匹配的成分时存在同种免疫的潜在风险，但目前还没有用于计数输注非RBC成分中残留红细胞（rRBC）的自动化、准确的检测方法。材料和方法对已获批用于计算CSF样本中RBC和WBC的自动化ADVIA 120脑脊液（CSF）检测试剂盒进行了优化和测试，以测量血小板浓缩液（PC）和血浆成分中的rRBC。结果优化了样品稀释度、孵育时间和试剂体积，以用于非RBC血液制品。即使在低rRBC计数下，该测定也是线性的（R2 = 0.99）。根据rRBC水平，试验内和试验间变异的变异系数（CV）分别为2.2 - 9.4%和2.6 - 14.9%。在自动测定和手动计数之间发现良好的相关性（r = 0.995），这被认为是金标准。使用自动化测定，血沉棕黄层血小板浓缩物（PC）中rRBC（计数/单位）的范围为275505 x 106，单采PC中为1361 x 106。结论ADVIA CSF法是一种灵敏、准确的非RBC成分rRBC计数方法。
Background and Objectives There is no automated, accurate assay for the enumeration of residual red blood cells (rRBCs) in non-RBC components for transfusion, despite the potential risk of allo-immunization when mismatched components are transfused. Materials and Methods The automated ADVIA 120 cerebrospinal fluid (CSF) assay, which is approved to count RBCs and WBCs in CSF samples, was optimized and tested to measure rRBC in platelet concentrate (PC) and plasma components. Results Sample dilution, incubation time and reagent volume were optimized for use with non-RBC blood products. The assay was linear (R2 = 0.99), even at low rRBCs counts. Intra- and inter-assay variation gave coefficients of variance (CV) between 2.2 and 9.4% and 2.6 and 14.9%, respectively, depending on rRBC levels. Good correlation (r = 0.995) was found between the automated assay and manual counting, which is considered the gold standard. Using the automated assay, the range of rRBCs (count/unit) in buffy-coat platelet concentrate (PCs) was 275505 x 106 and in apheresis PCs was 1361 x 106. Conclusion The ADVIA CSF assay is a sensitive, precise and accurate means to assess rRBC counts in non-RBC components.