Transferrin receptors: structure and function.
Transferrin receptors: structure and function.
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DOI：
10.1016/0006-2952(84)90447-7
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发表时间：
1984-03
影响因子：
5.8
通讯作者：
I. Trowbridge;R. Newman;D. Domingo;C. Sauvage
中科院分区：
文献类型：
--
作者：
I. Trowbridge;R. Newman;D. Domingo;C. Sauvage
单克隆抗体的出现和分子生物学技术的改进对现代生物学产生了巨大的影响。这一点在细胞表面的研究中表现得最为明显。直到最近，人们才获得了大多数细胞表面分子结构的基本信息，在了解它们的功能方面也没有取得什么进展。然而，现在由于这些新技术，这两个领域正在取得迅速进展。在这里，我们将回顾转铁蛋白受体的现状，然后关注我们自己正在进行的工作的两个特定方面，这两个方面都涉及使用针对转铁蛋白受体的单克隆抗体。大约两年前，我们获得了针对人类和小鼠细胞中阻断受体功能的转铁蛋白受体的单克隆抗体。我们将描述这些抗体对细胞生长的影响，并讨论这项工作对其他功能性细胞表面分子的更广泛影响。另一个主题是用荧光激活的细胞分选方法选择用总人类DNA转化后表面表达人转铁蛋白受体的小鼠L细胞。这些L细胞转化子具有有趣的特性，为克隆人类转铁蛋白受体基因提供了一种途径。这种方法可能普遍适用于分离已获得单克隆抗体的其他细胞表面分子的基因。
The advent of monoclonal antibodies and improved molecular biologic techniques have had a tremendous impact upon modern biology. Nowhere is this more apparent than in studies of the cell surface, Until recently, only rudimentary information about the structure of most cell surface molecules had been obtained and little progress had been made in understanding how they function. Now, however, as a result of these new technologies, rapid progress is being made in both areas. Here, we will review the current status of transferrin receptors and then focus on two particular aspects of our own ongoing work that both involve the use of monoclonal antibodies against transferrin receptors. About two years ago, we obtained monoclonal antibodies against transferrin receptors of both human and murine cells that block receptor function. The effects of these antibodies on cell growth in uim and in viuo will be described and the broader implications of this work for other functional cell surface molecules will be discussed. The other topic concerns the selection by fluorescence-activated cell sorting of mouse L cells that express the human transferrin receptor on their surface after transformation with total human DNA. These L cell transformants have interesting properties and provide an approach to cloning the human transferrin receptor gene. This methodology may have general applicability for the isolation of the genes of other cell surface molecules against which monoclonal antibodies have been obtained.