MALTOOLIGOSACCHARIDES AS CHIRAL SELECTORS FOR THE SEPARATION OF PHARMACEUTICALS BY CAPILLARY ELECTROPHORESIS
MALTOOLIGOSACCHARIDES AS CHIRAL SELECTORS FOR THE SEPARATION OF PHARMACEUTICALS BY CAPILLARY ELECTROPHORESIS
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DOI：
10.1021/ac00092a028
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发表时间：
1994-10-15
影响因子：
7.4
通讯作者：
NOVOTNY, MV
中科院分区：
文献类型：
--
作者：
SOINI, H;STEFANSSON, M;NOVOTNY, MV
在缓冲溶液中线性麦芽糊精低聚糖和某些药学上感兴趣的对映体化合物之间的络合可以导致分析上期望的手性识别。不同的麦芽糖糊精的能力进行了评估，导致对映体分离在各种条件下的毛细管电泳。通过对不同缓冲溶液和有机溶剂添加剂的电泳迁移率和选择性的测定，探讨了手性识别的机理。手性溶质与麦芽糊精螺旋实体的差异相互作用成为这种对映选择性的基础。这一观点得到了H-1-和C-13-NMR实验的进一步支持。西孟旦，布洛芬，华法林，酮洛芬对映体的优化分离证明与布洛芬在治疗水平的血清样品中的手性测定一起。
Complexation between the linear maltodextrin oligosaccharides and certain enantiomeric compounds of pharmaceutical interest in buffered solutions can lead to an analytically desirable chiral recognition. Different maltodextrins were assessed in their capacity to cause enantiomeric separations under various conditions of capillary electrophoresis. The mechanism of chiral recognition has been probed through electrophoretic mobility and selectivity measurements for different buffer solutions and organic solvent additives. A differential interaction of chiral solutes with the maltodextrin helical entities emerges as the basis of such enantioselectivity. This notion is further supported by H-1- and C-13-NMR experiments. Optimized separations of simendan, ibuprofen, warfarin, and ketoprofen enantiomers are demonstrated together with a chiral determination of ibuprofen in a blood serum sample at the therapeutic level.