Cytokeratin expression in hematological neoplasms: A tissue microarray study on 866 lymphoma and leukemia cases
Cytokeratin expression in hematological neoplasms: A tissue microarray study on 866 lymphoma and leukemia cases
复制标题
DOI：
10.1016/j.prp.2008.02.008
复制
发表时间：
2008-01-01
影响因子：
2.8
通讯作者：
Tzankov, Alexandar
中科院分区：
文献类型：
--
作者：
Adams, Heiner;Schmid, Patrik;Tzankov, Alexandar
细胞角蛋白（CK）的异常表达是已知的，偶尔发生在恶性淋巴瘤。单克隆小鼠抗人CK混合物CK 22识别具有广泛分子量的角蛋白多肽，可用于诊断来源不明的肿瘤。使用组织微阵列技术，我们使用CK 22检测了1059例淋巴瘤和急性白血病病例（涵盖最常见的疾病实体）的异常CK表达。总的来说，866例（80%）阵列病例可评价，13例阳性病例（1.5%）发现：230例霍奇金淋巴瘤中，（0.4%），浆细胞骨髓瘤1例，弥漫性大B细胞淋巴瘤326例中2例（0.6%），5/18例套细胞淋巴瘤（26%），3/70例小细胞淋巴瘤/慢性淋巴细胞白血病（4%），1/27例外周T细胞淋巴瘤，未另行说明（4%）。免疫组化染色多呈细颗粒状，阳性细胞总数仅在霍奇金淋巴瘤和浆细胞瘤中超过10%。除1例套细胞淋巴瘤外，所有CK 22阳性病例均表达特异性单纯上皮细胞CK 8，而不表达基底/复层上皮细胞CK 5/6。异常CK表达可在一小部分其他特征性B和T细胞淋巴瘤中遇到，但在急性白血病中不存在，这在难以鉴别诊断的情况下应予以考虑。(c)2008年Elsevier GmbH。All rights reserved.
Aberrant expression of cytokeratins (CK) is known to occasionally occur in malignant lymphomas. The monoclonal mouse-anti-human CK cocktail CK22 recognizes keratin polypeptides with a wide range of molecular weights and can be applied in diagnostic panels for tumors of unknown origin. Using tissue microarray technology, we tested 1059 lymphoma and acute leukemia cases, covering the most common disease entities, for aberrant CK expression, using CK22. In total, 866 of the arrayed cases were evaluable (80%), and 13 positive cases (1.5%) were found: I out of 230 Hodgkin lymphomas (0.4%), 1 plasina cell myeloma, 2 out of 326 diffuse large B-cell lymphomas (0.6%), 5 out or 18 mantle cell lymphomas (26%), 3 out or 70 small cell lymphomas/chronic lymphocytic leukemias (4%) and I out of 27 peripheral T-cell lymphomas, not otherwise specified (4%). Immunostaining was finely granular in most cases, and the total amount of positively staining cells exceeded 10% only in the cases of Hodgkin lymphoma and plasmocytoma. All CK22-positive cases, except for one mantle cell lymphoma, expressed the specific simple epithelial CK8 but not the basal/stratified epithelial CK5/6. Aberrant CK expression can be encountered in a small subset of otherwise characteristic B- and T-cell lymphomas, but not in acute leukemias, which should be considered in difficult differential diagnostic settings. (c) 2008 Elsevier GmbH. All rights reserved.