Evaluation of portal-systemic shunting in rats from mesenteric and splenic beds.
Evaluation of portal-systemic shunting in rats from mesenteric and splenic beds.
复制标题
大鼠肠系膜和脾床门体分流的评估。
DOI：
10.1007/bf01300758
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发表时间：
1991
影响因子：
3.1
通讯作者：
VanThiel,DH
中科院分区：
文献类型：
--
作者：
Stauber,RE;Ruthardt,FW;Tauxe,WN;VanThiel,DH
在部分门静脉结扎的大鼠中，当使用脾内注射微球来确定分流程度时，95±0.9%的脾血流从门静脉分流至体循环。尽管门体系统分流的程度如此之大，但门体系统分流的一些生化和内分泌后果的发生水平低于所发现的分流程度的预期水平。为了解决这些不一致的结果，在具有不同程度和/或持续时间的门静脉狭窄的麻醉门静脉高压大鼠中研究了从脾脏和肠系膜床的分流。肠系膜床的分流率平均为 66.7±29.9%（范围 5.1-99.1%），并且受到门静脉狭窄程度和持续时间的影响。相比之下，从脾床的分流率平均为 97.3±4.0%（范围 79-99.9%），并且根据门静脉狭窄程度确定，各组之间没有差异。脾床的分流始终大于肠系膜床的分流。肠系膜分流与血清胆汁酸水平相关，但与脾分流无关。肠系膜分流与体重调整后的肝脏质量和血清睾酮水平呈负相关。根据在门静脉高压大鼠中获得的这些数据，得出的结论是，脾脏注射微球高估了门静脉分流。相比之下，肠系膜注射微球产生的分流值与独立确定的分流的生化和内分泌后果密切相关。这些观察结果支持所获得的肠系膜分流测量的有效性。
In rats with partial portal vein ligation, 95±0.9% of the splenic blood flow is shunted from the portal to the systemic circulation when an intrasplenic injection of microspheres is used to determine the degree of shunting. Despite this magnitude of portal-systemic shunting, several biochemical and endocrine consequences of portal-systemic shunting occur at levels below what is expected for the degree of shunting found. In an effort to resolve these discordant findings, shunting from both the splenic and the mesenteric bed was studied in anesthetized portal hypertensive rats with various degrees and/or duration of portal vein stenosis. The shunting from the mesenteric bed averaged 66.7±29.9% (range 5.1–99.1%) and was influenced both by the degree and duration of portal vein stenosis. In contrast, shunting from the splenic bed averaged 97.3±4.0% (range 79–99.9%) and demonstrated no variation between groups determined by the degree of portal vein stenosis. The shunting from the splenic bed was consistently greater than that found from the mesenteric bed. Mesenteric but not splenic shunting correlated with serum bile acid levels. Mesenteric shunting was related inversely to the weight-adjusted liver mass and to serum testosterone levels. Based upon these data obtained in portal hypertensive rats, it is concluded that splenic injections of microspheres overestimate portal-systemic shunting. In contrast, mesenteric injections of microspheres yield values for shunting that correlate well with independently determined biochemical and endocrine consequences of shunting. These observations support the validity of the mesenteric shunting measurements obtained.