Microbiome Dynamics in a Shrimp Grow-out Pond with Possible Outbreak of Acute Hepatopancreatic Necrosis Disease.
Microbiome Dynamics in a Shrimp Grow-out Pond with Possible Outbreak of Acute Hepatopancreatic Necrosis Disease.
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虾长大的池塘中的微生物组动力学，可能爆发急性肝癌坏死疾病。
DOI：
10.1038/s41598-017-09923-6
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发表时间：
2017-08-24
影响因子：
4.6
通讯作者：
Wang HC
中科院分区：
文献类型：
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作者：
Chen WY;Ng TH;Wu JH;Chen JW;Wang HC
急性肝胰腺坏死病（Acute hepatopancreatic necrosis disease，AHPND）是对虾养殖区常见的一种高死亡率疾病。尽管已知AHPND是由携带质粒相关PirABvp毒素基因的致病性副溶血性弧菌引起的，但尚未研究微生物组干扰的影响。本研究采用Illumina测序技术，对放养白色仔虾后23 ~ 37 d AHPND发育期的62个虾池样品进行了16 SrRNA基因的测序。在此期间，池塘海水和对虾胃中的微生物群落经历了不同的动态演替。尽管有PirABvp的拷贝，但主坐标分析揭示了与AHPND相关的胃微生物组变化的两个不同阶段。AHPND显著改变了胃中的细菌多样性;它在大约7天内将香农指数降低了53.6%，改变了以弧菌和芽孢杆菌为优势种群的微生物组，并改变了种间相互作用网络的连通性和复杂性。引起AHPND的弧菌属物种被预测为与多个寄生和过境成员共同出现在蓝细菌、杆状病毒和蓝细菌中。这项研究对AHPND感染期间微生物组动态的见解对于在虾养殖池塘中最大限度地减少这种疾病可能是有价值的。
Acute hepatopancreatic necrosis disease (AHPND) (formerly, early mortality syndrome) is a high-mortality-rate shrimp disease prevalent in shrimp farming areas. Although AHPND is known to be caused by pathogenic Vibrio parahaemolyticus hosting the plasmid-related PirABvp toxin gene, the effects of disturbances in microbiome have not yet been studied. We took 62 samples from a grow-out pond during an AHPND developing period from Days 23 to 37 after stocking white postlarvae shrimp and sequenced the 16S rRNA genes with Illumina sequencing technology. The microbiomes of pond seawater and shrimp stomachs underwent varied dynamic succession during the period. Despite copies of PirABvp, principal co-ordinates analysis revealed two distinctive stages of change in stomach microbiomes associated with AHPND. AHPND markedly changed the bacterial diversity in the stomachs; it decreased the Shannon index by 53.6% within approximately 7 days, shifted the microbiome with Vibrio and Candidatus Bacilloplasma as predominant populations, and altered the species-to-species connectivity and complexity of the interaction network. The AHPND-causing Vibrio species were predicted to develop a co-occurrence pattern with several resident and transit members within Candidatus Bacilloplasma and Cyanobacteria. This study’s insights into microbiome dynamics during AHPND infection can be valuable for minimising this disease in shrimp farming ponds.