Deleted viral RNAs and lymphocytic choriomeningitis virus persistence in vitro.
Deleted viral RNAs and lymphocytic choriomeningitis virus persistence in vitro.
复制标题
删除病毒 RNA 和淋巴细胞性脉络丛脑膜炎病毒在体外的持久性。
DOI：
10.1099/0022-1317-69-8-1893
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Southern,PJ
中科院分区：
文献类型：
--
作者：
Francis,SJ;Southern,PJ
大多数组织培养细胞系的淋巴细胞性脉络丛脑膜炎病毒（LCMV）感染导致非致细胞病变的持续感染。体外持续性感染与小鼠持续性LCMV感染有许多共同特征;两者都与感染性病毒滴度降低、病毒糖蛋白在感染细胞表面的有限积累和干扰颗粒的产生有关。我们已经使用凝胶电泳和杂交技术来分析LCMV基因的表达在持续感染的一些组织培养细胞系。我们的研究表明，尽管在体外持续性LCMV感染过程中可以检测到缺失的病毒RNA，但缺失的RNA与持续性之间可能没有必然的联系。此外，我们发现，LCMV干扰活性可以在明显缺失的细胞内病毒RNA的情况下产生。
Lymphocytic choriomeningitis virus (LCMV) infection of most tissue culture cell lines results in a non-cytopathic persistent infection. Persistent infectionsin vitroshare many characteristics with persistent LCMV infection of mice; both are associated with decreased titres of infectious virus, restricted accumulation of viral glycoproteins at the surface of infected cells and the generation of interfering particles. We have used gel electrophoresis and hybridization techniques to analyse LCMV gene expression during persistent infection of a number of tissue culture cell lines. Our study has demonstrated that, although deleted viral RNAs can be detected during persistent LCMV infectionin vitro, there may not be an obligatory association between deleted RNAs and persistence. In addition, we have found that LCMV interfering activity can be produced in the apparent absence of deleted intracellular viral RNAs.