A two-photon fluorescent probe for intracellular detection of tyrosinase activity.
A two-photon fluorescent probe for intracellular detection of tyrosinase activity.
复制标题
DOI：
10.1002/asia.201200762
复制
发表时间：
2012-12
期刊：
影响因子：
--
通讯作者：
Shengyong Yan;Rong Huang;Changcheng Wang;Yimin Zhou;Jia-Qi Wang;Bo-Shi Fu;X. Weng;Xiang Zhou
中科院分区：
文献类型：
--
作者：
Shengyong Yan;Rong Huang;Changcheng Wang;Yimin Zhou;Jia-Qi Wang;Bo-Shi Fu;X. Weng;Xiang Zhou
AAN有效传感器：合成了双光子荧光探针NHU，用于体外和黑色素瘤细胞中酪氨酸酶活性的光学检测。NHU由4-氨基苯酚部分和萘胺单元组成，两者通过脲键连接。当暴露于酪氨酸酶时，4-氨基苯酚位点逐渐氧化为相应的邻醌，最终释放出高荧光产物6-酰基-N-甲基-2-萘胺（AAN）。
AAN effective sensor: The two-photon turn-on fluorescent probe NHU was synthesized to optically detect tyrosinase activity in vitro and in melanoma cells. NHU is composed of a 4-aminophenol moiety and a naphthylamine unit, both of which are connected through a urea linkage. Upon exposure to tyrosinase, the 4-aminophenol site is gradually oxidized to the corresponding orthoquinone, ultimately releasing the highly fluorescent product 6-acyl-N-methyl-2-naphthylamine (AAN).