The interaction between MtDELLA-MtGAF1 complex and MtARF3 mediates transcriptional control of MtGA3ox1 to elaborate leaf margin formation in Medicago truncatula.
The interaction between MtDELLA-MtGAF1 complex and MtARF3 mediates transcriptional control of MtGA3ox1 to elaborate leaf margin formation in Medicago truncatula.
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MtDELLA-MtGAF1 复合物和 MtARF3 之间的相互作用介导 MtGA3ox1 的转录控制，以阐述蒺藜苜蓿叶缘的形成。
DOI：
10.1093/pcp/pcaa163
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发表时间：
2021
影响因子：
4.9
通讯作者：
Zhou Chuanen
中科院分区：
文献类型：
--
作者：
Wen Lizhu;Kong Yiming;Wang Hongfeng;Xu Yiteng;Lu Zhichao;Zhang Jing;Wang Minmin;Wang Xiao;Han Lu;Zhou Chuanen
叶片形状多样性的分子机制一直是研究人员的极大兴趣。叶的形状取决于锯齿的样式和叶缘的凹陷程度。多个转录因子和激素信号参与了这一过程。在这项研究中，我们的特点是小锯齿叶（SSL）的发展作用，通过分析模式豆科植物蒺藜苜蓿的隐性突变体。拟南芥GA 3-氧化酶1（GA 3 ox 1）的直系同源物MtGA 3 ox 1/SSL是GA生物合成所必需的。MtGA 3 ox 1功能的丧失导致植株和侧生器官变小。mtga 3 ox 1突变体的突出表型是更明显的叶缘，表明GA水平在叶缘形成中的关键作用。此外，35 S：MtDELLA 2 Δ DELLA和35 S：MtARF 3转基因植株的叶片边缘呈深波浪状，这与mtga 3 ox 1的叶片边缘相似。进一步的研究表明，MtGA 3 ox 1受MtDELLA 1/2/3-MtGAF 1复合物依赖性反馈调节的控制。同时，MtARF 3作为MtDELLA 2/3-MtGAF 1复合物的竞争性抑制剂，间接抑制MtGA 3 ox 1的表达。这些研究结果表明，GA反馈调节电路在叶缘形成中起着重要作用，其中转录因子之间的翻译后相互作用是一个额外的功能。
The molecular mechanisms underlying diversity of leaf shapes have been of great interest to researchers. Leaf shape depends on the pattern of serrations and the degree of indentation of leaf margins. Multiple transcription factors and hormone signaling are involved in this process. In this study, we characterized the developmental roles of SMALL AND SERRATED LEAF (SSL) by analyzing a recessive mutant in the model legume Medicago truncatula. An ortholog of Arabidopsis thaliana GA3-oxidase 1 (GA3ox1), MtGA3ox1/SSL, is required for GA biosynthesis. Loss of function in MtGA3ox1 results in the small plant and lateral organs. The prominent phenotype of the mtga3ox1 mutant is the more pronounced leaf margin, indicating the critical role of GA level in leaf margin formation. Moreover, 35S: MtDELLA2 ΔDELLAand 35S: MtARF3 transgenic plants display leaves with the deeply wavy margin, which resembles those of mtga3ox1. Further investigations show that the MtGA3ox1 is under the control of MtDELLA1/2/3-MtGAF1 complexes-dependent feedback regulation. Meanwhile, MtARF3 behaves as a competitive inhibitor of MtDELLA2/3-MtGAF1 complexes to repress the expression of MtGA3ox1 indirectly. These findings suggest that GA feedback regulatory circuits play a fundamental role in leaf margin formation, in which the posttranslational interaction between transcription factors functions as an additional feature.