Effects of Sodium Trimetaphosphate, Associated or Not with Fluoride, on the Composition and pH of Mixed Biofilms, before and after Exposure to Sucrose
Effects of Sodium Trimetaphosphate, Associated or Not with Fluoride, on the Composition and pH of Mixed Biofilms, before and after Exposure to Sucrose
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DOI：
10.1159/000501262
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发表时间：
2020-12-01
期刊：
影响因子：
4.2
通讯作者：
Botazzo Delbem, Alberto Carlos
中科院分区：
文献类型：
--
作者：
Cavazana, Thamires Priscila;Pessan, Juliano Pelim;Botazzo Delbem, Alberto Carlos
本研究的目的是评估三偏磷酸钠（TMP），与氟（F），对氟，钙（Ca），磷（P）的浓度和对混合生物膜的pH值的影响，变形链球菌和白色念珠菌，暴露于蔗糖之前和之后。生物膜在三种TMP浓度（0.25、0.5或1%）下接受三种处理（它们开始形成后72、78和96小时），有或没有500 ppm的F。含有500和1,100 ppm F的溶液以及人工唾液也作为对照进行了测试。生物膜的pH值进行了测量和F，Ca和P的浓度进行了测定（固相和液相）。在平行实验中，在第三次处理（96 h）后，将生物膜暴露于20%蔗糖溶液以模拟致龋挑战，并测定培养基的pH、F、Ca、P和TMP。数据通过双因素ANOVA提交，然后进行Fisher最小显著性差异检验（p < 0.05）。用TMP和500 ppm F处理导致生物膜流体中较高的F浓度。虽然TMP不影响Ca浓度，但单独用TMP处理的生物膜呈现出更高的P浓度。用1%TMP和F处理导致生物膜的最高pH值，在致龋挑战之前和之后。得出结论，TMP增加生物膜中的F和P，并且其存在促进培养基的pH增加，即使在致龋挑战之后。
The aim of the present study was to evaluate the influence of sodium trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose. The biofilms received three treatments (72, 78, and 96 h after the beginning of their formation), at three TMP concentrations (0.25, 0.5, or 1%), with or without F at 500 ppm. Solutions containing 500 and 1,100 ppm F as well as artificial saliva were also tested as controls. Biofilm pH was measured and the concentrations of F, Ca, and P were determined (solid and fluid phases). In a parallel experiment, after the third treatment (96 h), the biofilms were exposed to a 20% sucrose solution to simulate a cariogenic challenge and the pH of the medium, F, Ca, P, and TMP were determined. The data were submitted by two-way ANOVA, followed by Fisher's least significant difference test (p < 0.05). Treatment with TMP and 500 ppm F led to higher F concentration in the biofilm fluid. Although TMP did not affect Ca concentrations, biofilms treated with TMP alone presented higher P concentrations. Treatment with 1% TMP and F led to the highest pH values of the biofilm, both before and after the cariogenic challenge. It was concluded that TMP increases F and P in the biofilm and that its presence promotes an increase in the pH of the medium, even after the cariogenic challenge.