Differential sensitivity of mouse hematopoietic stem cells to merocyanine 540.
Differential sensitivity of mouse hematopoietic stem cells to merocyanine 540.
复制标题
小鼠造血干细胞对部花青540的敏感性差异。
DOI：
10.1111/j.1432-0436.1981.tb01129.x
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Spivak,JL
中科院分区：
文献类型：
--
作者：
Sieber,F;Meagher,RC;Spivak,JL
在体内和体外克隆试验的未成熟小鼠血细胞表明，不同群体的造血祖细胞有很大的不同，就其敏感性的光动力损伤所造成的荧光染料Mercury 540。晚期红系祖细胞是最敏感的细胞，其次是多能干细胞、早期红系祖细胞和粒细胞/巨噬细胞祖细胞，敏感性依次降低。所有有核骨髓细胞中只有约2%-4%被Mercury 540染色，这与小鼠骨髓中祖细胞的当前频率估计值相关性良好。我们的研究结果表明，Mercury结合位点的表达是发育调节，因此，可能提供一个有用的分子标记的血细胞分化和造血祖细胞的有效纯化的基础。
In vivo and in vitro clonal assays of immature mouse blood cells showed that diffferent populations of hematopoietic progenitor cells differ considerably with respect to their sensitivity to photodynamic damages caused by the fluorescent dye Merocyanine 540. Late erythroid progenitors were the most sensitive cells followed in order of decreasing sensitivity by pluripotent stem cells, early erythroid progenitors, and granulocyte/macrophage progenitors. Only about 2%–4% of all nucleated marrow cells were stained with Merocyanine 540 which correlated well with current frequency estimates of progenitor cells in mouse bone marrow. Our findings indicate that the expression of Merocyanine binding sites is developmentally regulated and might, therefore, provide a useful molecular marker for blood cell differentiation and a basis for an effective purification of hematopoietic progenitor cells.