QTL Analysis of Aluminum Resistance in Rice (Oryza sativa L.)
QTL Analysis of Aluminum Resistance in Rice (Oryza sativa L.)
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DOI：
10.1007/s11104-006-9086-3
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发表时间：
2006-08
期刊：
影响因子：
4.9
通讯作者：
Y. Xue;J. Wan;Ling Jiang;Linglong Liu;N. Su;H. Zhai;Jian Feng Ma
中科院分区：
文献类型：
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作者：
Y. Xue;J. Wan;Ling Jiang;Linglong Liu;N. Su;H. Zhai;Jian Feng Ma
铝毒性被认为是酸性土壤中水稻产量低下的主要原因之一。本研究利用水稻耐铝品种Asominori （Oryza sativaL.）和铝敏感品种IR24 （O. sativaL.）杂交的重组自交系和完整的连锁图谱，分析了控制铝抗性的相对根伸长（RRE）数量性状位点。在第1、9、11号染色体上共检测到qRRE-1、qRRE-9、qrre -11 3个qtl，其LOD评分范围为2.64 ~ 3.60，表型变异解释范围为13.5 ~ 17.7%。Asominori等位基因在所有三个qtl上都与Al抗性相关。利用IR24遗传背景（IAS）中的Asominori染色体片段代换系（CSSLs）证实了这些qtl的存在。通过QTL比较分析，1号染色体和9号染色体上的两个QTL （qrre -1和qrre -9）在不同水稻群体中表现出一致性，而eqrre -11则是新发现的，与玉米10号染色体上的一个主要抗铝基因是同源的。这一区域可能为分离不同谷物抗铝机制相关基因提供了重要依据。这些结果也为利用标记辅助选择（MAS）和qtl构建提高水稻抗铝能力提供了可能。
Aluminum (Al) toxicity is considered as one of the primary causes of low-rice productivity in acid soils. In the present study, quantitative trait loci (QTLs) controlling Al resistance based on relative root elongation (RRE) were dissected using a complete linkage map and a recombinant inbred lines (RILs) derived from a cross of Al-tolerantjaponicacultivar Asominori (Oryza sativaL.) and Al-sensitiveindicacultivar IR24 (O. sativaL.). A total of three QTLs (qRRE-1, qRRE-9, andqRRE-11) were detected on chromosomes 1, 9, and 11 with LOD score ranging from 2.64 to 3.60 and the phenotypic variance explained from 13.5 to 17.7%. The Asominori alleles were all associated with Al resistance at all the three QTLs. The existence of these QTLs was confirmed using Asominori chromosome segment substitution lines (CSSLs) in IR24 genetic background (IAS). By QTL comparative analysis, the two QTLs (qRRE-1andqRRE-9) on chromosomes 1 and 9 appeared to be consistent among different rice populations whileqRRE-11was newly detected and syntenic with a major Al resistance gene on chromosome 10 of maize. This region may provide an important case for isolating genes responsible for different mechanisms of Al resistance among different cereals. These results also provide the possibilities of enhancing Al resistance in rice breeding program by marker-assisted selection (MAS) and pyramiding QTLs.