Improved method for cannula fixation for long-term intracerebral brain infusion
Improved method for cannula fixation for long-term intracerebral brain infusion
复制标题
长期脑内输注插管固定方法的改进
DOI：
10.1016/j.jneumeth.2017.07.026
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发表时间：
2017
影响因子：
3
通讯作者：
Pahnke J
中科院分区：
文献类型：
--
作者：
Sike Á;Wengenroth J;Upīte J;Brüning T;Eiriz I;Sántha P;Biverstål H;Jansone B;Haugen HJ;Krohn M;Pahnke J
背景植入式渗透微型泵通常通过连接到导管和套管上用于长期的、脑靶向递送各种实验药物。在立体定向手术过程中，插管必须在x-y方向上正确放置，并且还必须相对于z方向上的注射点（深度）正确放置。然而，现有的插管保持器的平坦固定基座不允许容易地、牢固地固定到弯曲形状的颅骨上。新方法我们已经开发了一种改进的方法，结果描述了颅骨硅胶垫片的应用及其在实验室中快速、可靠的生产，并与现有方法进行了比较。强力胶或水泥目前被用作首选方法。然而，弯曲形状的颅骨表面不能很好地与平坦且刚性的插管适配器配合，这导致随着时间的推移的固定问题，从而导致宽的输注通道，并且通常还导致从脑内施加的药剂朝向表面脑膜的泄漏问题。作为不适当的固定的另一个后果，套管可能会从头骨松动实验结束前或它会导致脑组织损伤，伤害动物，导致整个实验的失败。ConclusionsThe易于生产的间隔有利于长期的关键步骤，立体定向脑灌注实验与脑内导管在一个高度安全和可重复的方式。
BackgroundImplanted osmotic minipumps are commonly used for long-term, brain-targeted delivery of a wide range of experimental agents by being connected to a catheter and a cannula. During the stereotactical surgery procedure, the cannula has to be placed correctly in the x-y directions and also with respect to the injection point in the z-direction (deepness). However, the flat fixation base of available cannula holders doesn’t allow an easy, secure fixation onto the curve-shaped skull.New methodWe have developed a modified method for a better fixation of the cannula holder by using an easy-to-produce, skull-shaped silicone spacer as fixation adapter.ResultsWe describe the application and its fast and reliable production in the lab.Comparison with existing method(s)Superglue or cement is currently being used as the method of choice. However, the curve-shaped skull surface does not fit well with the flat and rigid cannula adapter which leads to fixation problems over time causing wide infusion channels and often also to leakage problems from intracerebrally applied agents towards the surface meninges. As another consequence of the inappropriate fixation, the cannula may loosen from the skull before the end of the experiment or it causes damage to the brain tissue, harming the animals with leading to a failure of the whole experiment.ConclusionsThe easy-to-produce spacer facilitates the crucial step of long-term, stereotactic brain infusion experiments with intracerebral catheters in a highly secure and reproducible way.
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DOI：
10.1523/jneurosci.17-19-07288.1997
发表时间：
1997-10
期刊：
The Journal of Neuroscience
影响因子：
--
作者：
Karen S. Chen;Merry C. Nishimura;M. Armanini;C. Crowley;S. Spencer;H. Phillips
通讯作者：
Karen S. Chen;Merry C. Nishimura;M. Armanini;C. Crowley;S. Spencer;H. Phillips
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DOI：
--
发表时间：
1997
期刊：
Alcoholism, clinical and experimental research.
影响因子：
--
作者：
Ullman,MD;Ventura,RF;Draski,LJ;Deitrich,RA;Baker,RC
通讯作者：
Baker,RC
DOI：
10.2353/ajpath.2008.071176
发表时间：
2008-09
期刊：
The American journal of pathology
影响因子：
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作者：
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通讯作者：
Dun-Sheng Yang;Asok Kumar;Philip H. Stavrides;J. Peterson;Corrine M Peterhoff;M. Pawlik;E. Levy;A. Cataldo;R. Nixon
大鼠脑室内注射加巴喷丁可增加氟草酯癫痫发作阈值
影响因子：
2.5
作者：
Joseph Oommen;Alli C. Kraus;R. Fisher
通讯作者：
R. Fisher
影响因子：
4.8
作者：
Raposinho, PD;Pedrazzini, T;Aubert, ML
通讯作者：
Aubert, ML