Experimental study on the PAR-1 expression around hemotoma following intracerebral hemorrhage in rats
Experimental study on the PAR-1 expression around hemotoma following intracerebral hemorrhage in rats
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DOI：
10.1007/bf02832008
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Guan Jingxia;Sun Shenggang;Cao Xuebing;Chen Zhibin
中科院分区：
文献类型：
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作者：
Guan Jingxia;Sun Shenggang;Cao Xuebing;Chen Zhibin
为探讨脑出血后血肿周围PAR-1mRNA和蛋白的表达及其与凝血酶的关系，采用大鼠右侧尾状核立体定向注射VII型胶原酶。RT-PCR法检测PAR-1mRNA表达，免疫组化法检测PAR-1蛋白表达。结果发现，脑出血后6h血肿周围PAR-1mRNA和蛋白表达增强(P<；0.05)，2d达高峰(P<；0.01)，然后下降。凝血酶脑内注射后PAR-1mRNA和蛋白表达的变化规律与脑出血相似。水飞蓟素组PAR-1mRNA和蛋白的表达与对照组无显著差异。提示脑出血后PAR-1mRNA和蛋白表达明显增加，可能与凝血酶密切相关。PAR-1表达上调可能参与了凝血酶诱导的神经毒性损伤。
In order to explore the PAR-1 mRNA and protein expression around hemotoma following intracerebral hemorrhage and the relation between the PAR-1 expression and thrombin, collagenase VII was stereotaxically injected into right caudate nucleus in rats. The PAR-1 mRNA expression was detected by RT-PCR method and the PAR-1 protein expression by immunohistochemical method respectively. It was found that the PAR-1 mRNA and protein expression around hemotoma was increased at 6 h after intracerebral hemorrhage (P<0.05), peaked at 2 days (P<0.01), and then declined. The change pattern of the PAR-1 mRNA and protein expression was similar to that of intracerebral hemorrhage after thrombin intracerebral injection. The PAR-1 mRNA and protein expression in hirudin group showed no significant, difference with control group. These results indicated that the PAR-1 mRNA and protein expression were markedly increased after intracerebral hemorrhage, which may be closely related to thrombin. Upregulation of the PAR-1 expression may involve in neurotoxic injury induced by thrombin.