Role of NMDA receptors in the increase of glucose metabolism in the rat brain induced by fluorocitrate
Role of NMDA receptors in the increase of glucose metabolism in the rat brain induced by fluorocitrate
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DOI：
10.1016/j.neulet.2007.01.031
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发表时间：
2007-03-30
影响因子：
2.5
通讯作者：
Inoue, Osamu
中科院分区：
文献类型：
--
作者：
Hirose, Shinichiro;Umetani, Yukiko;Inoue, Osamu
本文研究了向大鼠右侧纹状体内注入氟柠檬酸盐（FC，1 nmol/μ l，2 μ l）抑制胶质细胞代谢对葡萄糖代谢的影响。通过组织解剖法和放射自显影法测定，在FC输注后4 h，观察到右侧大脑皮质和纹状体中[F-18]氟脱氧葡萄糖（[F-18]FDG）摄取（45 min）显著增加。在纹状体中未观察到[F-18]FDG的初始摄取（I min）显著增加。用N-甲基-d-天冬氨酸（NMDA）受体拮抗剂地佐环平（MK-801）预处理，不仅在FC灌注的半球，而且在对侧半球（盐水灌注侧），[18 F]FDG摄取减少。NIK-801未改变[18F]FDG注射后1、5和45 min血浆中的放射性浓度。在输注FC的大鼠脑中观察到MK-801对葡萄糖代谢的影响，这与之前的报告不同，之前的报告表明正常大鼠脑某些区域的葡萄糖代谢增加。此外，MK-801预处理几乎完全消除了FC诱导的纹状体葡萄糖代谢增强。在大脑皮层，右半球与左半球放射性浓度的相对比值仍保持在1.37（组织解剖法）或1.55（放射自显影法），表明MK-801部分阻断了FC促进该区域葡萄糖代谢的作用。这些结果表明，NMDA介导的信号传递在抑制胶质细胞代谢引起的葡萄糖利用增加中起重要作用。(c)2007年由Elsevier爱尔兰有限公司出版。
The effect of inhibition of,I glial metabolism by infusion of fluorocitrate (FC, I nmol/mu 1, 2 [mu l) into the right striatum of the rat brain on the glucose metabolism was studied. Significant increases in [F-18]fluorodeoxyglucose ([F-18]FDG) uptake (45 min) in the right cerebral cortex and striatum were observed 4h after the infusion of FC, both as determined by the tissue dissection method and autoradiography. No significant increase in the initial uptake of [F-18]FDG (I min) was seen in the striatum. Pretreatment with dizocilpine (MK-801), an N-methyl-d-aspartate (NMDA) receptor antagonist, reduced [18F]FDG uptake in not only FC infused hemisphere but also in the contralateral hemisphere (saline-infused side). The radioactivity concentrations in plasma at 1, 5 and 45 min after the [18F]FDG injection were not altered by NIK-801. This effect of MK-801 on glucose metabolism m observed in the rat brain infused with FC was different from previous reports which indicated an increase in glucose metabolism in some areas of normal rat brain. In addition, the enhancement of glucose metabolism in the striatum induced by FC was almost completely abolished by pretreatment with MK-801. In the cerebral cortex, the relative ratio of radioactivity concentration in the right hemisphere to that in the left hemisphere still remained 1.37 (tissue dissection method) or 1.55 (autoradiography), which indicated that MK-801 partially blocked the effect of FC of enhancing glucose metabolism in this region. These results indicate an important role of NMDA-mediated signal transmission on the increase Of glucose utilization induced by inhibition of glial metabolism. (c) 2007 Published by Elsevier Ireland Ltd.