LTP2 hypomorphs show genotype-by-environment interaction in early seedling traits in Arabidopsis thaliana.
LTP2 hypomorphs show genotype-by-environment interaction in early seedling traits in Arabidopsis thaliana.
复制标题
LTP2 亚型在拟南芥幼苗早期性状中表现出基因型与环境的相互作用。
DOI：
10.1101/2023.05.11.540469
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Queitsch,Christine
中科院分区：
文献类型：
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作者：
Alexandre,CristinaM;Bubb,KerryL;Schultz,KarlaM;Lempe,Janne;Cuperus,JoshT;Queitsch,Christine
同基因个体可以表现出看似随机的表型差异，限制了基因型对表型预测的准确性。这种表型变异的程度部分取决于遗传背景，这就引发了关于控制随机表型变异的基因的问题。我们关注拟南芥早期幼苗性状，发现角质层相关基因脂质转移蛋白2（LTP 2）的亚型极大地增加了幼苗表型的变异，包括下胚轴长度，向重力性和角质层渗透性。许多tp2下胚轴明显短于野生型下胚轴，而其他的则与野生型相似。具有长下胚轴和短下胚轴的tp2幼苗之间表皮特性和基因表达的差异表明，角质层完整性的丧失是所观察到的表型变异的主要决定因素。我们发现环境条件可以揭示或掩盖ltp 2亚型变异的增加，并发现其最接近的旁系同源物LTP1的表达增加是ltp 2表型的必要条件。
Isogenic individuals can display seemingly stochastic phenotypic differences, limiting the accuracy of genotype‐to‐phenotype predictions. The extent of this phenotypic variation depends in part on genetic background, raising questions about the genes involved in controlling stochastic phenotypic variation.Focusing on early seedling traits inArabidopsis thaliana, we found that hypomorphs of the cuticle‐related geneLIPID TRANSFER PROTEIN 2(LTP2) greatly increased variation in seedling phenotypes, including hypocotyl length, gravitropism and cuticle permeability. Manyltp2hypocotyls were significantly shorter than wild‐type hypocotyls while others resembled the wild‐type.Differences in epidermal properties and gene expression betweenltp2seedlings with long and short hypocotyls suggest a loss of cuticle integrity as the primary determinant of the observed phenotypic variation. We identified environmental conditions that reveal or mask the increased variation inltp2hypomorphs and found that increased expression of its closest paralogLTP1is necessary forltp2phenotypes.Our results illustrate how decreased expression of a single gene can generate starkly increased phenotypic variation in isogenic individuals in response to an environmental challenge.