ANTIBODIES TO 2 MAJOR CHICKEN HEAT-SHOCK PROTEINS CROSS-REACT WITH SIMILAR PROTEINS IN WIDELY DIVERGENT SPECIES
ANTIBODIES TO 2 MAJOR CHICKEN HEAT-SHOCK PROTEINS CROSS-REACT WITH SIMILAR PROTEINS IN WIDELY DIVERGENT SPECIES
复制标题
DOI：
10.1128/mcb.2.3.267
复制
发表时间：
1982-01-01
影响因子：
5.3
通讯作者：
SCHLESINGER, MJ
中科院分区：
文献类型：
--
作者：
KELLEY, PM;SCHLESINGER, MJ
纯化了鸡胚成纤维细胞热休克诱导蛋白中的三个，并制备了兔抗它们的抗体。这些抗体被用于放射免疫沉淀反应和固相免疫测定，以检测非热休克鸡组织和广泛不同物种[酵母]提取物中的抗原物质（酿酒酵母）、黏菌（Dictyosteelium discoideum），甲藻（多甲藻），玉米苗，蠕虫（秀丽隐杆线虫）、果蝇胚胎、爪蟾肾细胞系、小鼠L929细胞、人WI 38细胞和红细胞血影]。抗体2的主要鸡热休克蛋白，chsp89和chsp70，交叉反应的蛋白质类似MW在正常的胚胎和成年鸡组织和提取物中广泛不同的生物。这些数据提供了进一步的证据的普遍性的热休克反应和保护蛋白质诱导的这种类型的压力。
Three of the proteins induced by heat shock of chicken embryo fibroblasts were purified, and rabbit antibodies were raised against them. These antibodies were used in radioimmune precipitation reactions and in a solid-phase immune assay to detect antigentic material in non-heat-shocked chicken tissues and in extracts of widely different species [yeast (Saccharomyces cerevisiae), slime mold (Dictyostelium discoideum), dinoflagellate (Peridinium cinctum), corn seedling, worm (Caenorhabditis elegans), Drosophila melanogaster embryo, Xenopus kidney cell line, mouse L929 cells, human WI38 cells and erythrocyte ghosts]. Antibodies to 2 of the major chicken heat shock proteins, chsp89 and chsp70, cross-reacted with proteins of similar MW in normal embryonic and adult chicken tissues and in extracts from widely different organisms. These data provide further evidence for the universality of the heat shock response and conservation of proteins induced by this type of stress.